Progesterone Antibody (XM207) [mFluor Violet 450 SE]

• Reactivity: Hu
• Applications: ELISA
• Clone: XM207
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: mFluor Violet 450 SE

Progesterone Antibody (XM207) [mFluor Violet 450 SE] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with purified progesterone conjugated with BSA.
• Localization: Secreted
• Specificity: Progesterone, Ka = 3.0 x 10^9_ M^-1. Not cross-reacting with BSA. Cross-reactivity (%): Progesterone (100); 17-Hydroxyprogesterone (1.0); 11-Hydroxyprogesterone (25); 5-alpha-pregnane-3,20 dionene (10.5); Corticosterone (0.01); Pregnenolone (0.9); Deoxycorticosterone (0.30); Deoxycortisol (0.03); Cortisol (0.002)
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity: Ion exchange chromatography

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product NB110-7982. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Ion exchange chromatography

Notes

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Progesterone Antibody (XM207) [mFluor Violet 450 SE]

• 4-Pregnen-3,20-dione
• Progesterone

Background

Progesterone plays a central role in the reproductive events associated with the establishment and maintenance of pregnancy. Progesterone receptor, a member of the steroid receptor superfamily, mediates the physiologic effects of progesterone. The PGR gene uses separate promoters and translational start sites to produce 2 isoforms, PRA and PRB, which are identical except for an additional 165 amino acids present only in the N terminus of PRB. Although PRA and PRB share several structural domains, they are distinct transcription factors that mediate their own response genes and physiologic effects with little overlap. It is composed of three domains: a modulating N terminal domain, a DNA binding domain and a C terminal steroid binding domain. Progesterone levels 1. men 30-60 pg/0.1ml 2. women pre ovulatory phase: 20-160 pg/0.1ml; ovulatory phase: 1,000-1,700 pg/0.1ml; post ovulatory phase: 1,000-1,700 pg/0.1ml; Pregnant: 16-18 weeks: 300-800 pg/0.1ml; 28-30 weeks: 6,500-14,700 pg/0.1ml; 38-40 weeks: 12,000-19,000 pg/0.1ml.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.