POMK/SGK196 Antibody (6F10)

Western Blot: FLJ23356 Antibody (6F10) [H00084197-M03] - Analysis of FLJ23356 expression in HepG2 (Cat # L019V1).

Immunocytochemistry/Immunofluorescence: FLJ23356 Antibody (6F10) [H00084197-M03] - Analysis of monoclonal antibody to FLJ23356 on HeLa cell. Antibody concentration 10 ug/ml

Western Blot: FLJ23356 Antibody (6F10) [H00084197-M03] - Western blot analysis of FLJ23356 over-expressed 293 cell line, cotransfected with FLJ23356 Validated Chimera RNAi or non-transfected control. Blot probed with H00084197-M03. GAPDH(36.1 kDa)used as loading control.

Western Blot: FLJ23356 Antibody (6F10) [H00084197-M03] - Analysis of FLJ23356 expression in HeLa(Cat # L013V1).

Western Blot: FLJ23356 Antibody (6F10) [H00084197-M03] - Analysis of FLJ23356 expression in transfected 293T cell line by FLJ23356 monoclonal antibody (M03), clone 6F10. Lane 1: FLJ23356 transfected lysatE (38.5 KDa). Lane 2: Non-transfected lysate.

ELISA: FLJ23356 Antibody (6F10) [H00084197-M03] - Detection limit for recombinant GST tagged FLJ23356 is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, KD
• Clone: 6F10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

POMK/SGK196 Antibody (6F10) Summary

• Immunogen: FLJ23356 (NP_115613, 251 a.a. ~ 350 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GDFVAPEQLWPYGEDVPFHDDLMPSYDEKIDIWKIPDISSFLLGHIEGSDMVRFHLFDIHKACKSQTPSERPTAQDVLETYQKVLDTLRDAMMSQAREML
• Specificity: FLJ23356 - hypothetical protein FLJ23356
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: POMK
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Knockdown Validated
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, RNAi validation and ELISA. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 24556084)

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for POMK/SGK196 Antibody (6F10)

• FLJ23356
• MGC126597
• protein kinase-like protein SgK196
• Sugen kinase 196

Background

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. STKL subfamily.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for POMK/SGK196 Antibody (H00084197-M03)(1)

Publication using H00084197-M03

• von Renesse A, Petkova MV, Lutzkendorf S et al. POMK mutation in a family with congenital muscular dystrophy with merosin deficiency, hypomyelination, mild hearing deficit and intellectual disability. J. Med. Genet. 2014-02-21 [PMID: 24556084] (IHC-Fr, WB, ICC/IF, Human)

Bioinformatics

• Gene Symbol: POMK
• Entrez:
  • 84197(Human)
• Uniprot:
  • NP_115613(Human)