Polyoma Virus, Large T Antigen Antibody (PyLT) - BSA Free

• Reactivity: V-Vi
• Applications: WB, ICC/IF, IP
• Clone: PyLT
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Polyoma Virus, Large T Antigen Antibody (PyLT) - BSA Free Summary

• Description: Novus Biologicals Rat Polyoma Virus, Large T Antigen Antibody (PyLT) - BSA Free (NB100-2755) is a monoclonal antibody validated for use in WB, ICC/IF and IP. Anti-Polyoma Virus, Large T Antigen Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Polyoma virus-transformed Wistar rat fibroblast cell line Py REWA5/T1A1.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:100 - 1:2000
• Theoretical MW: 100 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Reactivity reported in scientific literature (PMID: 25278611).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Background

The mouse polyoma virus (Py) is an oncogenic virus, which encodes for three early proteins, large, middle and small T (tumour) antigen. PyLT allows isolation of viral T antigens.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Polyoma Virus, Large T Antigen Antibody (NB100-2755)(3)

Publications using NB100-2755

• Theurillat JP, Udeshi ND, Errington WJ et al. Ubiquitylome analysis identifies dysregulation of effector substrates in SPOP-mutant prostate cancer. Science 2014-10-03 [PMID: 25278611] (Western Blot, Human)
• Theurillat Jean-Philippe P, Udeshi Namrata D, Errington Wesley J et al. Ubiquitylome analysis identifies dysregulation of effector substrates in SPOP-mutant prostate cancer Science 2014 Oct 03 [PMID: 25278611] (WB, Human)
• Theurillat Jean-Philippe P, Udeshi Namrata D, Errington Wesley J et al. Prostate cancer. Ubiquitylome analysis identifies dysregulation of effector substrates in SPOP-mutant prostate cancer. Science. 2014 Oct 03 [PMID: 25278611] (WB, Human)