![ELISA: Pollen allergen Phl p 1 Antibody (Clone 25) [NBP2-81275] - Binding curve of Pollen allergen Phl p 1 Antibody (Clone 25) [NBP2-81275] to Phl p 1 ELISA Plate coated with Phl p 1 at a concentration of 2 ug/ml. A 3-fold serial dilution from 10,000 to 0.1 ng/ml was performed using NBP2-81276. For detection, a 1:4000 dilution of HRP-labelled goat anti-human kappa antibody was used.](http://images.novusbio.com/fullsize/Pollen-allergen-Phl-p-1-Antibody-Clone-25-ELISA-NBP2-81275-img0001.jpg "ELISA: Pollen allergen Phl p 1 Antibody (Clone 25) [NBP2-81275] - Binding curve of Pollen allergen Phl p 1 Antibody (Clone 25) [NBP2-81275] to Phl p 1 ELISA Plate coated with Phl p 1 at a concentration of 2 ug/ml. A 3-fold serial dilution from 10,000 to 0.1 ng/ml was performed using NBP2-81276. For detection, a 1:4000 dilution of HRP-labelled goat anti-human kappa antibody was used.")
| Reactivity | PlSpecies Glossary |
| Applications | WB, DB, ELISA |
| Clone | Clone 25 |
| Clonality | Monoclonal |
| Host | Human |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Recombinant Phl p 1, a grass pollen allergen. |
| Specificity | This antibody binds to the Timothy grass allergen Phl p 1 and shows cotss reactivity with natural group 1 allergens from a variety of grass species such as oat, sweet vernal grass, Bermuda grass, rye grass, common reed, Kentucky blue grass, rye, wheat and maize (weak). |
| Isotype | IgE Kappa |
| Clonality | Monoclonal |
| Host | Human |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This full-length, reformatted human antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques. This antibody binds to Phl p 1 and competes with up to 43.4% (mean 19.8%) of IgE from allergic patient serum. |
| Storage | Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C. |
| Buffer | PBS |
| Preservative | 0.02% Proclin 300 |
| Concentration | 1 mg/ml |
| Purity | Affinity purified |
Secondary Antibodies |
Isotype Controls |
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![Western Blot: Goat anti-Human IgG Fc Secondary Antibody [NB7446] - Image from verified customer review. Image using the HRP form of this antibody.](https://images.novusbio.com/images/Goat-anti-Human-IgG-Fc-Secondary-Antibody-Western-Blot-NB7446-img0001.jpg "Western Blot: Goat anti-Human IgG Fc Secondary Antibody [NB7446] - Image from verified customer review. Image using the HRP form of this antibody.")