Pma1 Antibody (40B7)

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Product Details

Summary
Reactivity YeSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
40B7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Pma1 Antibody (40B7) Summary

Immunogen
Generated from a sequence of yeast Pma1 [Swiss-Prot# P05030]
Localization
Plasma Membrane
Marker
Plasma Membrane Marker
Isotype
IgG
Clonality
Monoclonal
Host
Mouse
Gene
Pma1
Purity
Tissue culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:1000-1:5000
  • Immunohistochemistry 1:1000-1:5000
  • Western Blot 1:10000
Application Notes
This Pma1 Antibody (40B7) is useful for Immunocytochemistry/Immunofluorescence and Western blot analysis. For Western blots of yeast protein samples (cell lysates), use at a dilution of 1:10,000, followed by chemiluminescent detection (ECL). For non-ECL western detection methods, use at a dilution of 1:1,000 to 1:5,000. The predicted molecular weight by western blot is 100 kDa.
Theoretical MW
100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NB120-4645.

Reactivity Notes

Saccharomyces cerevisiae.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Supplied as concentrated hybridoma cell culture media.
Preservative
5mM Sodium Azide
Purity
Tissue culture supernatant

Alternate Names for Pma1 Antibody (40B7)

  • Plasma membrane ATPase 1
  • PMA1
  • Proton pump 1
  • YGL008C

Background

Pma1 (plasma membrane H+-ATPase) belongs to cation transport ATPase (P-type) (TC 3.A.3) family and is an essential protein in yeast/Saccharomyces cerevisiae wherein it pumps out H+ for controlling intracellular pH as well as for generating an electrochemical gradient required to drive cellular import of metabolites (this activity utilizes cellular ATP). Glucose acts as an inducer of Pma1 expression and its catalytic activity through phosphorylation at Ser-899, Thr-912 and Ser-911 sites. Upon glucose starvation, Pma1 is negatively regulated by casein kinase I (Yck1p and Yck2p) phosphorylation and glucose mediated Pma1 activation requires glucose sensor Snf3p, G-protein Gpa2p and transport/phosphorylation of glucose. Hsp30 negatively regulate Pma1 activity during heat shock conditions and contributes towards thermo-tolerance by lowering cellular ATP consumption. Pma1 expression has been shown to induce oncogenic transformation of NIH 3T3 cells and cause increased intracellular pH. Pma1 has emerged as a potential target for development of anti-fungal agents, its suitability as a biomarker for toxicity mediated stress and as a model for study of membrane protein biogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Pma1 Antibody (NB120-4645)(1)

We have publications tested in 1 confirmed species: Yeast.


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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Pma1 Antibody (NB120-4645) (0)

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Secondary Antibodies

 

Isotype Controls

Additional Pma1 Products

Array NB120-4645

Bioinformatics Tool for Pma1 Antibody (NB120-4645)

Discover related pathways, diseases and genes to Pma1 Antibody (NB120-4645). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Bioinformatics

Gene Symbol Pma1