Phosphoserine Antibody [Biotin]

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Product Details

Summary
Reactivity NASpecies Glossary
Applications WB, ELISA, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Biotin

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Phosphoserine Antibody [Biotin] Summary

Immunogen
This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with phosphoserine conjugated KLH.
Specificity
The antibody reacts with free phosphoserine, phosphoserine conjugated to carriers such as thyroglobulin or BSA, and detects the presence of phosphoserine in proteins of both unstimulated and stimulated cell lysates. It shows minimal reactivity by ELISA and western blotting with proteins containing phosphotyrosine or phosphothreonine
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA 1:5000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
This affinity-purified antibody is suitable for use in ELISA and western blotting assays. The antibody reacts specifically with phosphoserine and shows minimal reactivity by ELISA and western blotting with proteins containing phosphotyrosine or phosphothreonine residues. The antibody reacts with free phosphoserine, phospho-serine conjugated - carriers such as thyroglobulin or BSA, and detects the presence of phosphoserine in proteins of both unstimulated and stimulated cell lysates. Although not tested, this antibody is likely functional in RIA, flow cytometry and immunohistochemistry.
Readout System

Reactivity Notes

Reactive in phosphoserine

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 40% (v/v) Glycerol
Preservative
No Preservative
Purity
Immunogen affinity purified

Alternate Names for Phosphoserine Antibody [Biotin]

  • Phosphoserine; Phosphothreonine

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional Phosphoserine Products

Phosphoserine NBP1-77727

Research Areas for Phosphoserine Antibody (NBP1-77727)

Find related products by research area.

Blogs on Phosphoserine.

Phosphotyrosine: A Global Player in Human Health and Disease
Protein tyrosine phosphorylation is a fundamental mechanism for controlling many aspects of cellular processes, as well as aspects of human health and diseases. Compared with phosphoserine and phosphothreonine, phosphotyrosine signaling is more tightl...  Read full blog post.

New Techniques Using Phosphoserine Antibodies
Phosphoserine, the phosphorylated modification of the amino acid serine, is a central post-translational modification within a cell for many biological and biomedical processes. The phosphorylation of specifically four residue types - histidine, serin...  Read full blog post.

Phosphotyrosine: Affecting Locally Acting Globally
Over the past two decades, it has become clear that tyrosine phosphorylation plays a pivotal role in a variety of important signaling pathways in multicellular organisms. In the typical vertebrate cell, phosphotyrosine represents only a tiny fraction...  Read full blog post.

Phosphoserine: A Basic Cellular Signal with Wide Ranging Effects
 Phosphoserine is an ester of serine and phosphoric acid which results from posttranslational modifications. Phosphorylation is a key post-translational modification necessary for normal cellular signaling. Phosphorylated proteins mediate cell divisio...  Read full blog post.

Phosphotyrosine is Critical Signal Transduction and Regulation
Phosphotyrosine is the phosphorylated version of the amino acid tyrosine, which results from the activation of intracellular protein kinases (e.g. via growth factors) during normal growth and development, well as in transformation and oncogenesis. Pho...  Read full blog post.

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