Penicillin Antibody (Pen-9) [CoraFluor™ 1]

CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.

• Reactivity: All-NA
• Applications: ELISA
• Clone: Pen-9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: CoraFluor 1

Penicillin Antibody (Pen-9) [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
  
  CoraFluor(TM) 1, amine reactive
  
  CoraFluor(TM) 1, thiol reactive
  
  For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
• Immunogen: Penicilloyl-transferrin conjugate
• Localization: Depends on the target
• Specificity: This antibody reacts mainly with the hiazolidine ring of penicillin, but not with the lactam ring. May react with the fused beta lactam/thiazolidine ring of the penicilloyl group and would appear sensitive to modification of the shared N atom of the lactam/thiazolidine ring by substitution or conjugation. It appears insensitive to the structure of the side chain of the penicilloyl group. This antibody reacts with the following penicillin; Benzylpenicillin, Ampicillin, Amoxicillin and 6-Aminopenicillanic acids. Penicillin is a group of Beta-lactam antibiotics used in the treatment of bacterial infections caused by susceptible, usually Gram-positive, organisms. -lactam antibiotics work by inhibiting the formation of peptidoglycan cross-links in the bacterial cell wall, which results in cytolysis. This antibody is useful in the study of allergy to penicillin.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Benzylpenicillin

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A or G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for Penicillin Antibody (Pen-9) [CoraFluor™ 1]

• Ampicillin
• Benzylpenicillin
• dicloxacillin
• flucloxacillin
• methicillin
• PCN
• Penicilin
• Penicillin G
• Phenoxymethylpenicillin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.