Pdi1p Antibody (38H8)

Images

 
Western Blot: Pdi1p Antibody (38H8) [NBP1-05391] - Western Blot analysis of whole yeast protein extracts detected Pdi1p. Molecular weight detected is 58.2 kDa.

Product Details

Summary
Reactivity YeSpecies Glossary
Applications WB, ICC/IF
Clone
38H8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Pdi1p Antibody (38H8) Summary

Immunogen
Yeast membrane preparations
Localization
endoplasmic reticulum and nuclear envelope
Marker
Endoplasmic Reticulum Marker
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Tissue culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:500-1:1000
Application Notes
This Pdi1p (38H8) antibody is useful in Western blot and Immunocytochemistry/Immunofluorescence. In WB of yeast protein extracts a ~65 kDa band can be seen. In ICC/IF using yeast cells, endoplasmic reticulum staining can be seen.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Yeast.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
10mM Sodium Azide
Purity
Tissue culture supernatant

Alternate Names for Pdi1p Antibody (38H8)

  • MFP 1
  • MFP1
  • PDI 1
  • PDI
  • PDI1
  • Protein disulfide isomerase
  • Thioredoxin related glycoprotein 1
  • TRG 1
  • TRG1

Background

One of the important aspects of secretory protein's folding process is the formation of native disulfide bonds which is catalyzed by enzyme protein disulfide isomerase (PDI) and yeast Saccharomyces cerevisiae has about 5 protein disulfide isomerase (PDI) family members (EUG1, MPD1, MPD2, EPS1 and PDI1/PDI1p). Pdi1p is a typical member of its family which is an essential gene and is considered homologus to human PDI with no significant differences; however, the two proteins are only 29% identical. It is a major ER protein with chaperon/co-chaperon activity and is involved in the retention of resident ER proteins as well as in recognizing proteins targeted for ER-associated degradation (ERAD). Yeast Pdi1p is a folding catalyst which introduces disulfide bonds into its substrates and rearranges or reduces pre-existing ones, and besides its oxidoreductase activity, Pdi1p acts as a molecular chaperone that also facilitates folding of proteins which do not contain disulfide bonds.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Pdi1p Antibody (NBP1-05391) (0)

There are no publications for Pdi1p Antibody (NBP1-05391).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Pdi1p Antibody (NBP1-05391) (0)

There are no reviews for Pdi1p Antibody (NBP1-05391). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Pdi1p Antibody (NBP1-05391) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Pdi1p Antibody (38H8) and receive a gift card or discount.