Pdi1p Antibody (38H8)


Western Blot: Pdi1p Antibody (38H8) [NBP1-05391] - Western Blot analysis of whole yeast protein extracts detected Pdi1p. Molecular weight detected is 58.2 kDa.

Product Details

Reactivity YeSpecies Glossary
Applications WB, ICC/IF, IP

Order Details

Pdi1p Antibody (38H8) Summary

Yeast nuclear preparations
endoplasmic reticulum and nuclear envelope
Endoplasmic Reticulum Marker
Tissue culture supernatant
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  • Immunocytochemistry/Immunofluorescence 1:500-1:1000
  • Immunoprecipitation
  • Western Blot 1:10000
Application Notes
This Pdi1p (38H8) antibody is useful in Western blot and Immunocytochemistry/Immunofluorescence. In WB of yeast protein extracts a ~65 kDa band can be seen. In ICC/IF using yeast cells, endoplasmic reticulum staining can be seen.
Theoretical MW
58.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

S. cerevisiae

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Supplied as concentrated hybridoma cell culture media.
5mM Sodium Azide
Tissue culture supernatant

Alternate Names for Pdi1p Antibody (38H8)

  • MFP 1
  • MFP1
  • PDI 1
  • PDI
  • PDI1
  • Protein disulfide isomerase
  • Thioredoxin related glycoprotein 1
  • TRG 1
  • TRG1


One of the important aspects of secretory protein's folding process is the formation of native disulfide bonds which is catalyzed by enzyme protein disulfide isomerase (PDI) and yeast Saccharomyces cerevisiae has about 5 protein disulfide isomerase (PDI) family members (EUG1, MPD1, MPD2, EPS1 and PDI1/PDI1p). Pdi1p is a typical member of its family which is an essential gene and is considered homologus to human PDI with no significant differences; however, the two proteins are only 29% identical. It is a major ER protein with chaperon/co-chaperon activity and is involved in the retention of resident ER proteins as well as in recognizing proteins targeted for ER-associated degradation (ERAD). Yeast Pdi1p is a folding catalyst which introduces disulfide bonds into its substrates and rearranges or reduces pre-existing ones, and besides its oxidoreductase activity, Pdi1p acts as a molecular chaperone that also facilitates folding of proteins which do not contain disulfide bonds.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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