PCGF3 Antibody

Western Blot: PCGF3 Antibody [NB100-1171] - (1ug/ml) of Human Heart lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

• Reactivity: Hu, Mu, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

PCGF3 Antibody Summary

• Description: Novus Biologicals Goat PCGF3 Antibody (NB100-1171) is a polyclonal antibody validated for use in WB and ELISA. Anti-PCGF3 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-PLLLHYRPKMDLL corresponding to C-Terminus according to NP_006306.2.
• Specificity: This antibody is expected to recognise the 247 aa isoform only (NP_006306), not the 132 aa isoform (NP_694982).
• Predicted Species: Mouse (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PCGF3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:2000
  • Western Blot 0.5 - 2 ug/ml
• Application Notes: WB: Approx. 35 kDa band observed in human heart lysate (predicted MW of approx. 27 kDa band according to NP_006306).
• Control:

  Human Heart Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PCGF3 Antibody

• DKFZp686D20235
• DONG1
• FLJ36550
• FLJ43813
• MGC129615
• MGC40413
• polycomb group ring finger 3
• polycomb group RING finger protein 3
• ring finger protein 3
• RNF3
• RNF3ARING finger protein 3A

Background

The protein encoded by this gene contains a C3HC4 type RING finger, which is a motif known to be involved in protein-protein interactions. The specific function of this protein has not yet been determined

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PCGF3 Antibody (NB100-1171)(1)

Publication using NB100-1171

• Freemont PS. The RING finger. A novel protein sequence motif related to the zinc finger. Ann N Y Acad Sci 1993-06-11 [PMID: 8317827]

Bioinformatics

• Gene Symbol: PCGF3
• Entrez:
  • 10336(Human)
  • 69587(Mouse)
• Uniprot:
  • NP_006306.(Human)