PCDHAC2 Antibody (3D12)

Western Blot: PCDHAC2 Antibody (3D12) [H00056134-M03] - Analysis of PCDHAC2 expression in transfected 293T cell line by PCDHAC2 monoclonal antibody (M03), clone 3D12. Lane 1: PCDHAC2 transfected lysatE (96.2 KDa). Lane 2: Non-transfected lysate.

Western Blot: PCDHAC2 Antibody (3D12) [H00056134-M03] - Western blot analysis of PCDHAC2 over-expressed 293 cell line, cotransfected with PCDHAC2 Validated Chimera RNAi or non-transfected control. Blot probed with H00056134-M03. GAPDH(36.1 kDa) used as loading control.

Product Discontinued

PCDHAC2 Antibody (3D12) Summary

• Immunogen: PCDHAC2 (NP_061722, 81 a.a. ~ 190 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. HLGAPSPRYLELDLTSGALFVNERIDREALCEQRPRCLLSLEVLAHNPVAVSAVEVEILDINDNSPRFPRPNYQLQVSESVAPGARFHIESAQDPDVGANSVQTYELSPS
• Specificity: PCDHAC2 - protocadherin alpha subfamily C, 2
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PCDHAC2
• Purity: Ascites

Applications/Dilutions

• Dilutions:
  • ELISA
  • RNA Inhibition
  • Western Blot 1:500RNAi Validation
• Application Notes: Antibody reactive against recombinant protein for western blot. It has also been used for RNAi validation and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: Ascites

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PCDHAC2 Antibody (3D12)

• MGC71598
• PCDH-ALPHA-C2
• protocadherin alpha subfamily C, 2
• protocadherin alpha-C2

Background

This gene is a member of the protocadherin alpha gene cluster, one of three related gene clusters tandemly linked on chromosome five that demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The alpha gene cluster is composed of 15 cadherin superfamily genes related to the mouse CNR genes and consists of 13 highly similar and 2 more distantly related coding sequences. The tandem array of 15 N-terminal exons, or variable exons, are followed by downstream C-terminal exons, or constant exons, which are shared by all genes in the cluster. The large, uninterrupted N-terminal exons each encode six cadherin ectodomains while the C-terminal exons encode the cytoplasmic domain. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins that most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been observed and additional variants have been suggested but their full-length nature has yet to be determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PCDHAC2
• Entrez:
  • 56134(Human)
• OMIM:
  • 606321(Human)
• Uniprot:
  • NP_061722(Human)