Pax3 Antibody (274212) [Unconjugated]

Images

 
Pax3 was detected in immersion fixed B16‑F1 mouse melanoma cell line using Mouse Anti-Human/Mouse Pax3 Monoclonal Antibody (Catalog # MAB2457) at 2 µg/mL for 3 hours at room temperature. Cells were stained ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, CyTOF-ready, Flow, ICC/IF
Clone
274212
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

Pax3 Antibody (274212) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Pax3 (isoform Pax3a)
Met1-Ser215
Accession # NP_000429
Specificity
Detects human and mouse Pax3 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
PAX3
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
MAB2457 in the following applications:

Publications
Read Publications using
MAB2457 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Pax3 Antibody (274212) [Unconjugated]

  • CDHS
  • HUP2
  • HUP2MGC120384
  • MGC120381
  • MGC120382
  • MGC120383
  • MGC134778
  • paired box 3
  • paired box gene 3 (Waardenburg syndrome 1)
  • paired box homeotic gene 3
  • paired box protein Pax-3
  • paired domain gene 3
  • paired domain gene HuP2
  • Pax3
  • Waardenburg syndrome 1
  • WS1
  • WS3

Background

Pax3 belongs to the family of paired box transcription factors. Pax family proteins typically contain a paired box domain and a paired-type homeodomain. These transcription factors play critical roles during fetal development. Human and mouse Pax3 share 98% amino acid sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Pax3 Antibody (MAB2457)(10)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: Functional Assay, ICC, IHC, Western Blot.


Filter By Application
Functional Assay
(1)
ICC
(3)
IHC
(1)
Western Blot
(4)
All Applications
Filter By Species
Human
(6)
Mouse
(3)
All Species
Showing Publications 1 - 10 of 10.
Publications using MAB2457 Applications Species
L Zhang, J Avery, A Yin, AM Singh, TS Cliff, H Yin, S Dalton Generation of Functional Brown Adipocytes from Human Pluripotent Stem Cells via Progression through a Paraxial Mesoderm State Cell Stem Cell, 2020;0(0):. 2020 [PMID: 32783886] (IHC, Human) IHC Human
V Sahakyan, E Pozzo, R Duelen, J Deprest, M Sampaolesi Methotrexate and Valproic Acid Affect Early Neurogenesis of Human Amniotic Fluid Stem Cells from Myelomeningocele Stem Cells Int, 2017;2017(0):6101609. 2017 [PMID: 29056972] (ICC, Human) ICC Human
Arts N, Cane S, Hennequart M, Lamy J, Bommer G, Van den Eynde B, De Plaen E microRNA-155, induced by interleukin-1ss, represses the expression of microphthalmia-associated transcription factor (MITF-M) in melanoma cells. PLoS ONE, 2015;10(4):e0122517. 2015 [PMID: 25853464] (Western Blot, Human) Western Blot Human
Abraham J, Nunez-Alvarez Y, Hettmer S, Carrio E, Chen H, Nishijo K, Huang E, Prajapati S, Walker R, Davis S, Rebeles J, Wiebush H, McCleish A, Hampton S, Bjornson C, Brack A, Wagers A, Rando T, Capecchi M, Marini F, Ehler B, Zarzabal L, Goros M, Michalek J, Meltzer P, Langenau D, LeGallo R, Mansoor A, Chen Y, Suelves M, Rubin B, Keller C Lineage of origin in rhabdomyosarcoma informs pharmacological response. Genes Dev, 2014;28(14):1578-91. 2014 [PMID: 25030697] (ICC, Human) ICC Human
Kikuchi K, Hettmer S, Aslam M, Michalek J, Laub W, Wilky B, Loeb D, Rubin B, Wagers A, Keller C Cell-cycle dependent expression of a translocation-mediated fusion oncogene mediates checkpoint adaptation in rhabdomyosarcoma. PLoS Genet, 2014;10(1):e1004107. 2014 [PMID: 24453992]
Plouhinec J, Roche D, Pegoraro C, Figueiredo A, Maczkowiak F, Brunet L, Milet C, Vert J, Pollet N, Harland R, Monsoro-Burq A Pax3 and Zic1 trigger the early neural crest gene regulatory network by the direct activation of multiple key neural crest specifiers. Dev Biol, 2014;386(2):461-72. 2014 [PMID: 24360906] (Functional Assay, Human) Functional Assay Human
Awaya , Tomonari, Kato , Takeo, Mizuno , Yuta, Chang , Hsi, Niwa , Akira, Umeda , Katsutsu, Nakahata , Tatsutos, Heike , Toshio Selective development of myogenic mesenchymal cells from human embryonic and induced pluripotent stem cells. PLoS ONE, 2012;7(12):e51638. 2012 [PMID: 23236522] (ICC, Human) ICC Human
Magli A, Schnettler E, Rinaldi F, Bremer P, Perlingeiro R Functional dissection of Pax3 in paraxial mesoderm development and myogenesis. Stem Cells, 2013;31(1):59-70. 2013 [PMID: 23081715] (Western Blot, Mouse) Western Blot Mouse
Gianakopoulos PJ, Mehta V, Voronova A, Cao Y, Yao Z, Coutu J, Wang X, Waddington MS, Tapscott SJ, Skerjanc IS MyoD directly up-regulates premyogenic mesoderm factors during induction of skeletal myogenesis in stem cells. J. Biol. Chem., 2011;286(4):2517-25. 2011 [PMID: 21078671] (Western Blot, Mouse) Western Blot Mouse
Gang EJ, Bosnakovski D, Simsek T, To K, Perlingeiro RC Pax3 activation promotes the differentiation of mesenchymal stem cells toward the myogenic lineage. Exp. Cell Res., 2008;314(8):1721-33. 2008 [PMID: 18395202] (Western Blot, Mouse) Western Blot Mouse

Review for Pax3 Antibody (MAB2457) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Other.

Reviews using MAB2457:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Other
(1)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
WB Other 02/19/2015
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Summary

ApplicationWestern Blot
Sample TestedSee PMID 21078671
SpeciesOther

Blocking

Blocking DetailsSee PMID 21078671

Details

Detection NotesSee PMID 21078671

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID 21078671

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Pax3 Antibody (MAB2457) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 IC2457U
Alexa Fluor 405 IC2457V
Alexa Fluor 488 IC2457G
Alexa Fluor 532 FAB2457X
Alexa Fluor 594 IC2457T
Alexa Fluor 647 IC2457R
Alexa Fluor 700 IC2457N
Alexa Fluor 750 IC2457S
Allophycocyanin IC2457A
Biotin FAB2457B
DyLight 350 FAB2457D
DyLight 405 FAB2457E
DyLight 488 FAB2457K
DyLight 550 FAB2457L
DyLight 594 FAB2457M
DyLight 650 FAB2457W
DyLight 680 FAB2457Y
DyLight 755 FAB2457Z
FITC FAB2457F
HRP FAB2457H
Janelia Fluor 549 FAB2457I
Janelia Fluor 646 FAB2457J
PerCP FAB2457C
Phycoerythrin IC2457P

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Blogs on Pax3.

FOXO1/FKHR (fork head in Rhabdomyosarcoma)
FOXO1 belongs to the very large Forkhead family of transcription factors which contain a conserved distinct DNA-binding domain known as the Forkhead Box, or FOX. The Forkhead domain is a 100 amino acid long motif capable of binding and bending DNA,...  Read full blog post.

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Recent Reviews

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Verified Customer
02/19/2015
Application: WB
Species: Other

Bioinformatics

Gene Symbol PAX3
Uniprot