Pancreatic trypsin inhibitor Antibody (18) - BSA Free

ELISA: Pancreatic trypsin inhibitor Antibody (18) - BSA Free [NBP2-23588] - The calibration curve of a sandwich assay for Aprotinin (low sensitivity) using NBP2-23590 as the capture antibody and NBP2-23587 as the biotinylated detection antibody.

ELISA: Pancreatic trypsin inhibitor Antibody (18) - BSA Free [NBP2-23588] - The calibration curve of a sandwich assay for Aprotinin (high sensitivity) using NBP2-23589 as the capture antibody and NBP2-23587 as the biotinylated detection antibody.

• Reactivity: Bv
• Applications: WB, ELISA
• Clone: 18
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Pancreatic trypsin inhibitor Antibody (18) - BSA Free Summary

• Description: Novus Biologicals Mouse Pancreatic trypsin inhibitor Antibody (18) - BSA Free (NBP2-23588) is a monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Aprotinin from bovine lung.
• Epitope: The epitope of Clone 18 is different from the epitopes of Clone 21 and Clone 35
• Specificity: Clone 18 binds bovine aprotinin immobilized on a solid phase or free in solution.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PTI
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Western Blot 1:100-1:2000
• Application Notes: ELISA: NBP2-23588 (as biotinylated detection antibody e.g. at 1 ug/mL) forms an antibody pair with NBP2-23589 (as capture antibody) for high-sensitivity sandwich ELISA of aprotinin at sample concentrations of 0.6-20 ug/mL. Aprotinin in urine diluted 1/1000 can be assayed against standards in buffer. For assay in plasma it is recommended to add aprotinin-free control plasma to the standards to match the plasma sample dilution. NBP2-23588 also forms an antibody pair with NBP2-23590 (as capture antibody) for sandwich ELISA of aprotinin at sample concentrations of 4-250 ug/mL. Both urine and plasma samples diluted 1/1000 can be assayed against standards in buffer. PBS-buffers are unsuitable for these assays and 0.2 M glycine sodium buffer, pH 8.8, is recommended. WB: NBP2-23588 can be used in Western blotting.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 10mM Phosphate (pH 7.4) and 0.5M NaCl
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Pancreatic trypsin inhibitor Antibody (18) - BSA Free

• Aprotinin
• Basic protease inhibitor
• BPI
• BPTI
• PTI pancreatic trypsin inhibitor
• PTI

Background

Aprotinin, also called bovine pancreatic trypsin inhibitor, is a single chain globular Kunitz-type serine proteinase inhibitor found in bovine pancreas, parotid gland and lung. It forms stable, enzymatically inactive complexes with trypsin, chymotrypsin, plasmin and kallikrein. Aprotinin has been used therapeutically as an antifibrinolytic agent under the name Trasylol (Bayer) to reduce blood loss during cardiopulmonary bypass surgery but this use has been associated with an increased frequency of acute renal injury

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PTI