pan Nuclear Pore Complex Marker Antibody (39C7)

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Immunocytochemistry/ Immunofluorescence: pan Nuclear Pore Complex Marker Antibody (39C7) [NBP1-05390] - man HeLa cells were stained with monoclonal antibody NBP1-05390, which binds to a nuclear pore complex antigen, and ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications ICC/IF
Clone
39C7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

pan Nuclear Pore Complex Marker Antibody (39C7) Summary

Immunogen
Yeast nuclear preparations
Specificity
Panspecific
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity
Tissue culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:50-1:500
Application Notes
This pan Nuclear Pore Complex Marker (39C7) antibody is useful for Immunocytochemistry/Immunofluorescence. For ICC/IF on yeast cells the recommended dilution is 1:100 to 1:500. On mammalian cells the recommended dilution is 1:50 to 1:100.

Reactivity Notes

Human, mouse and rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
No buffer
Preservative
10mM Sodium Azide
Purity
Tissue culture supernatant

Alternate Names for pan Nuclear Pore Complex Marker Antibody (39C7)

  • 107 kDa nucleoporin
  • NPC proteins
  • Nuclear pore complex protein Nup107
  • Nucleoporin 107kDa
  • Nucleoporin Nup107
  • NUP 107
  • NUP 84
  • NUP107
  • NUP84

Background

This monoclonal was raised by injecting mice with yeast nuclear preparations and screening the resulting hybridomas by immunofluorescence on yeast cells. 39C7 was one of a series of clones which strongly and specifically labeled the nuclear pore complex. When this antibody was tested on cells from other species, including rat, mouse and human cells, it has invariably strongly stained nuclear pore complexes, so it appears to be an excellent and panspecific marker for these important structures. 39C7 does not work well on western blots so we are currently unsure of the exact identity of the protein to which it binds.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

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