Pan Coronavirus Spike Antibody (38) - Azide and BSA Free

Western Blot: Pan Coronavirus Spike Antibody (38) [NBP3-14660] - Sample: Reocombinant Protein 10 ngLane 1: SARS-CoV-2 Spike S1-His Lane 2: SARS-CoV-2 Spike S1 NTD-Fc & AVI Lane 3: SARS-CoV-2 Spike S1 NTD-His & AVILane 4: SARS-CoV-2 Spike RBD-His Lane 5: SARS-CoV-2 Spike S2 ECD-His Lane 6: SARS-CoV-2 Spike S1+S2 ECD-His Lane 7: SARS-CoV Spike S1+S2 ECD-His Lane 8: MERS-CoV Spike S1+S2 ECD-His Lane 9: HCoV-NL63 Spike S1+S2 ECD-His Lane 10: HCoV-229E Spike S1+S2 ECD-His Lane 11: HCoV-HKU1 (isolate N5) Spike S1+S2 ECD-His Lane 12: HCoV-OC43 Spike S1+S2 ECD-His SecondaryGoat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.

• Reactivity: V-Vi
• Applications: WB, ELISA
• Clone: 38
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Pan Coronavirus Spike Antibody (38) - Azide and BSA Free Summary

• Description: This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Preservative-Free. Avoid repeated freeze-thaw cycles.
• Immunogen: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Coronavirus Spike S1+S2 ECD protein.
• Specificity: Recognizes the Spike of SARS-CoV-2, SARS-CoV, MERS-CoV, HCoV-NL63, HCoV-229E, HCoV-HKU1 (isolate N5), HCoV-OC43.
  
  Has cross-reactivity in ELISA and WB with:
  SARS-CoV-2 Spike S1+S2 ECD-His
  SARS-CoV-2 Spike S2 ECD-His
  SARS-CoV Spike S1+S2 ECD-His
  MERS-CoV Spike S1+S2 ECD-His
  HCoV-HKU1 (isolate N5) Spike S1+S2 ECD-His
  HCoV-229E Spike S1+S2 ECD-His
  HCoV-OC43 Spike S1+S2 ECD-His
  HCoV-NL63 Spike S1+S2 ECD-His
  
  No cross-reactivity in ELISA and WB with:
  SARS-CoV-2 Spike S1-His
  SARS-CoV-2 Spike S1 NTD-Fc & AVI
  SARS-CoV-2 Spike S1 NTD-His & AVI
  SARS-CoV-2 Spike RBD-His
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:1000-1:2000
  • Western Blot 1:1000-1:5000
• Application Notes: Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Protein A purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.