Oval Cell Marker Antibody (OC2-2F3) - BSA Free

Immunohistochemistry-Frozen: Oval Cell Marker Antibody (OC2-2F3) [NBP1-18964] - Immunohistochemical analysis of DDC treated mouse liver using NBP1-18964.

• Reactivity: Mu
• Applications: Flow, IHC
• Clone: OC2-2F3
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: This product is unpurified. The exact concentration of antibody is not quantifiable.

Oval Cell Marker Antibody (OC2-2F3) - BSA Free Summary

• Immunogen: Nonparenchymal cells from DDC-treated mice
• Localization: Cell surface
• Marker: Hepatic periductal cell Marker
• Specificity: Specific for proliferating hepatic duct cells.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Frozen 1:100
• Application Notes: This Oval Cell Marker (OC2-2F3) antibody is useful for Immunohistochemistry on acetone fixed frozen sections and Flow cytometry.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tissue culture supernatant
• Preservative: 0.05% Sodium Azide
• Concentration: This product is unpurified. The exact concentration of antibody is not quantifiable.
• Purity: Tissue culture supernatant

Background

Liver is well known to possess facultative stem cells which can be activated in response to injury and hepatic stem cell activation was first described in rat model, wherein, chemical damage in combination with partial hepatectomy resulted in the emergence of a new cell type which was described according to its nuclear morphology as "oval cells". The word 'oval cells' is now employed to define population of small proliferating cells with oval-shaped nuclei which arise within liver tissue following certain types of hepatic injury. Oval cell proliferation is activated when the proliferative capacity of hepatocytes is impaired, as well as in certain models of carcinogenesis. Histologically, hepatic tissue is highly complex and contains many different cell types including various kinds of hepatocytes, ducts, hematopoietic cells, endothelial cells, macrophages, stellate cells etc., and the oval cells have been proposed to be bipotential progenitor cells capable of generating both hepatocytes and bile ducts. Due to their bipotency and high clonogenicity, oval cells have been considered putative liver stem cells and research is in progress for elucidation of signaling molecules involved in controlling the activation, proliferation and differentiation of this unique cell population. Our oval cell antibodies have been developed against hepatic nonparenchymal cells (NPCs) from mice undergoing oval cell activation and with these antibodies, it is now possible to perform microarray analysis of distinct liver cell populations during induced oval cell response or certain pathological conditions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Oval Cell Marker Antibody (NBP1-18964)(1)

Publication using NBP1-18964

• Dorrell, C et al. Surface Markers for the Murine Oval Cell Response. Hepatology 48(0):1-10. 2008-01-01 [PMID: 18726953] (IHC-Fr, FLOW, Mouse)