O-GalNAc Antibody (9B9) - BSA Free

Western Blot analysis of Glycoconjugates showing detection of 67 kDa O-GalNAc protein using Mouse Anti-O-GalNAc Monoclonal Antibody, Clone 9B9 (NBP2-59309). Lane 1: Molecular Weight Ladder (MW). Lane 2: O-GalNAc-BSA. Lane 3: GalNAc-BSA. Lane 4: Galactose-BSA. Lane 5: Glucose-BSA. Load: 2.0 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-O-GalNAc Monoclonal Antibody (NBP2-59309) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-O-GalNAc Monoclonal Antibody, Clone 9B9 (NBP2-59309). Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-O-GalNAc Monoclonal Antibody (NBP2-59309) at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alexa Fluor 633 F-Actin stain. (C) O-GalNAc Antibody (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.

Analysis using Mouse Anti-O-GalNAc Monoclonal Antibody, Clone 9B9. Tissue: colon. Species: Mouse. Fixation: Formalin fixed, paraffin embedded. Primary Antibody: Mouse Anti-O-GalNAc Monoclonal Antibody at 1:25 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: Alexa Fluor 488. Counterstain: Actin-binding Phalloidin-Alexa Fluor 633; DAPI (blue) nuclear stain. Magnification: 63X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Alexa Fluor 633 F-Actin stain. (C) O-GalNAc Antibody (D) Composite

Tissue: Embryonic kidney cells (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-O-GalNAc Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). Courtesy of: Dr. Robert Burke, University of Victoria.

• Reactivity: All-NA
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 9B9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

O-GalNAc Antibody (9B9) - BSA Free Summary

• Immunogen: Synthetic B-O-Linked N-Acetylglucosamine conjugated to Keyhole Limpet Hemocyanin (KLH).
• Specificity: Specific for B-O-Linked N-Acetylgalactosamine and B-O-Linked N-Acetylglucosamine modified proteins. Does not cross-react with Glucose or Galactose modified proteins.
• Isotype: IgM
• Clonality: Monoclonal
• Host: Mouse
• Purity: PEG purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:1000
  • Immunocytochemistry/ Immunofluorescence 1:50
  • Immunohistochemistry 1:25
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Purity: PEG purified

Alternate Names for O-GalNAc Antibody (9B9) - BSA Free

• GalNAc
• O-linked N-acetylgalactosamine

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.