Nucleocapsid Antibody (1038729) [Unconjugated]

Western blot shows recombinant MERS-CoV Nucleocapsid protein. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Mouse MERS-CoV Nucleocapsid Monoclonal Antibody (Catalog # MAB10729) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for MERS-CoV Nucleocapsid at approximately 49 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Simple Western lane view shows recombinant MERS-CoV nucleocapsid, loaded at 0.2 mg/mL. A specific band was detected for Nucleocapsid at approximately 57 kDa (as indicated) using 25 µg/mL of Mouse Anti-MERS-CoV Nucleocapsid Monoclonal Antibody (Catalog # MAB10729) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: V
• Applications: WB, Simple Western
• Clone: 1038729
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Nucleocapsid Antibody (1038729) [Unconjugated] Summary

• Immunogen: Spodoptera frugiperda insect ovarian cell line Sf21-derived mouse MERS-CoV Nucleocapsid
  Met1-Thr411
  Accession # YP_007188586.1
• Specificity: Detects Mouse MERS-CoV Nucleocapsid in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Simple Western 25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Nucleocapsid Antibody (1038729) [Unconjugated]

• N Protein
• Nucleocapsid protein
• Nucleocapsid
• ORF9a protein

Background

MERS-CoV, which causes the Middles East Respiratory Syndrome (MERS), belongs to a family of viruses known as coronaviruses that are commonly comprised of four structural proteins: Spike protein (S), Envelope protein (E), Membrane protein (M), and Nucleocapsid protein (N) (1). While the S, E and M proteins build up the viral envelope, the N protein is involved transcription, replication and packaging of the viral RNA genome into a helical ribonucleocapsid (RNP) (1, 2). The MERS-CoV N protein is a ~45 kDa protein composed of two independent structural domains connected by a linker region. The N-terminal region contains an Intrinsically Disordered Region (3) and an RNA binding domain (4), the linker region interacts with the M protein and the C-terminal region contains a self-association domain (1, 2). The MERS-CoV N protein shares 46.3% and 4.5% amino acid sequence identity with SARS-CoV-1 and SARS-CoV-2 N protein, respectively. MERS-CoV N proteins have been shown to inhibit Type I Interferon(IFN) production(1). In addition, the N protein is an abundant protein during coronavirus infection and displays high immunogenic activity, making it a promising therapeutic target (5-7).

1. Li, Y. et al. (2019) Engineering. 5:940.
2. Hurst, K. R. et al. (2009) J. Virol. 83:7221.
3. Wang, Y. et al. (2015) Acta. Crystallogr. F. Struct. Biol. Commun. 71:977.
4. Papageorgiou, N. et al. (2016) Acta. Crystallogr. D. Struct. Biol. 72:192.
5. Che, X. Y. et al. (2004) J. Clin. Microbiol. 42:2629.
6. Guan, M. et al. (2004) Clin. Diagn. Lab. Immunol. 11:287.
7. Chang, C-K. et al.(2016) Drug Discov. Today. 21:562.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • YP_007188586.1()