Nuclear Antigen Antibody (NM106) - IHC-Prediluted

Immunohistochemistry-Paraffin: Nuclear Antigen Antibody (NM106) - IHC-Prediluted [NBP2-48213] - Formalin-fixed, paraffin-embedded Rat Lung stained with Pan Nuclear Antigen Antibody.

Immunohistochemistry-Paraffin: Nuclear Antigen Antibody (NM106) - IHC-Prediluted [NBP2-48213] - Formalin-fixed, paraffin-embedded human Tonsil stained with Pan Nuclear Antigen Antibody.

Immunohistochemistry-Paraffin: Nuclear Antigen Antibody (NM106) - IHC-Prediluted [NBP2-48213] - Formalin-fixed, paraffin-embedded human Tonsil stained with Pan Nuclear Antigen Antibody.

Immunohistochemistry-Paraffin: Nuclear Antigen Antibody (NM106) - IHC-Prediluted [NBP2-48213] - Formalin-fixed, paraffin-embedded Rat Colon stained with Pan Nuclear Antigen Antibody.

Immunofluorescentstaining of PFA-fixedHeLa cells with Nuclear Antigen Antibody (NM106) - IHC-Prediluted followed by goat anti-mouse IgG-CF488 (green). Membranes labeled with phalloidin (red).

• Reactivity: Hu, Mu, Rt
• Applications: IHC
• Clone: NM106
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: IHC-Prediluted

Nuclear Antigen Antibody (NM106) - IHC-Prediluted Summary

• Description: The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.
• Immunogen: Nuclei of HL60 cells
• Localization: Nuclei
• Marker: Pan-Nuclear Marker
• Specificity: This monoclonal antibody is an excellent marker for all nuclei in cells in tissues. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This monoclonal antibody recognizes an antigen associated with the nuclei in all cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
• Application Notes: Immunocytochemistry Acetone-fixed cells 0.25 - 0.5 ug/ml for 30 minutes at RT; Immunohistology Formalin-paraffin 0.25 - 0.5 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.

Reactivity Notes

Does not react with Porcine.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for Nuclear Antigen Antibody (NM106) - IHC-Prediluted

• Nuclei
• Nucleus

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.