Nuclear Antigen Antibody (NM106)

Immunocytochemistry/Immunofluorescence: Nuclear Antigen Antibody (NM106) [NBP2-45273] - Immunofluorescent staining of paraformaldehyde-fixed HeLa cells with Pan Nuclear Antigen Antibody followed by goat anti-Mouse IgG-CF488 (Green). Membranes are labeled with Phalloidin (Red).

Immunohistochemistry-Paraffin: Nuclear Antigen Antibody (NM106) [NBP2-45273] - Analysis of mouse testis tissue using Nuclear Antigen antibody. Primary antibody dilution: 0.5 ug/ml dilution. Image from verified customer review.

Immunohistochemistry-Paraffin: Nuclear Antigen Antibody (NM106) [NBP2-45273] - Human Tonsil stained with Pan Nuclear Antigen Antibody

Immunohistochemistry-Paraffin: Nuclear Antigen Antibody (NM106) [NBP2-45273] - Human Tonsil stained with Pan Nuclear Antigen Antibody.

Immunohistochemistry-Paraffin: Nuclear Antigen Antibody (NM106) [NBP2-45273] - Rat Colon stained with Pan Nuclear Antigen Antibody.

Immunohistochemistry-Paraffin: Nuclear Antigen Antibody (NM106) [NBP2-45273] - Rat Lung stained with Pan Nuclear Antigen Antibody.

• Reactivity: Hu, Mu, Rt
• Applications: Flow, ICC/IF, IHC
• Clone: NM106
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Nuclear Antigen Antibody (NM106) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-11613)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
• Immunogen: Nuclei of HL60 cells
• Localization: Nuclei
• Marker: Pan-Nuclear Marker
• Specificity: This monoclonal antibody is an excellent marker for all nuclei in cells in tissues. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This monoclonal antibody recognizes an antigen associated with the nuclei in all cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.

Reactivity Notes

Does not react with Porcine.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for Nuclear Antigen Antibody (NM106)

• Nuclei
• Nucleus

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for Nuclear Antigen Antibody (NBP2-45273) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer IHC-P Mouse 04/02/2022

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Testis
• Species: Mouse
• Lot: NM106

Comments

• Comments: 0.5 ug/ml dilution