![Immunocytochemistry/Immunofluorescence: Nuclear Antigen Antibody (235-1R) - Azide and BSA Free [NBP3-14102] - Immunofluorescence Analysis of MeOH-fixed HeLa cells with human Nuclear Antigen antibody (235-1R) followed by goat anti-mouse IgG-CF488 (green).](http://images.novusbio.com/fullsize/Nuclear-Antigen-Antibody-235-1R-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP3-14102-img0002.jpg "Immunocytochemistry/Immunofluorescence: Nuclear Antigen Antibody (235-1R) - Azide and BSA Free [NBP3-14102] - Immunofluorescence Analysis of MeOH-fixed HeLa cells with human Nuclear Antigen antibody (235-1R) followed by goat anti-mouse IgG-CF488 (green).")
| Reactivity | Hu, PmSpecies Glossary |
| Applications | Flow, ICC/IF, IHC, IP |
| Clone | 235-1R |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13912). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Nuclei of human myeloid leukemia biopsy cells |
| Localization | Nuclear |
| Marker | Human Cell Marker |
| Specificity | This monoclonal antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. monoclonal antibody235-1R recognizes an antigen associated with the nuclei in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations. |
| Isotype | IgG Kappa |
| Clonality | Monoclonal |
| Host | Rabbit |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | Immunocytochemistry (Acetone-fixed cells) (1-2ug/ml for 30 minutes at RT Immunohistology (Frozen): 1-2ug/ml for 30 minutes at RT Immunoprecipitation: 1-2 g/500ug protein lysate) Immunoprecipitation: 1-2 gram/500 ug protein lysate |
| Theoretical MW | 70,80 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10mM PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: Nuclear Antigen Antibody (235-1R) - Azide and BSA Free [NBP3-14102] - Immunofluorescence Analysis of PFA-fixed HeLa cells with human Nuclear Antigen antibody (235-1R) followed by goat anti-mouse IgG-CF488 (green).](http://images.novusbio.com/fullsize/Nuclear-Antigen-Antibody-235-1R-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP3-14102-img0001.jpg "Immunocytochemistry/Immunofluorescence: Nuclear Antigen Antibody (235-1R) - Azide and BSA Free [NBP3-14102] - Immunofluorescence Analysis of PFA-fixed HeLa cells with human Nuclear Antigen antibody (235-1R) followed by goat anti-mouse IgG-CF488 (green).")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
