Nsr1p Antibody (31C4)

Western Blot: Nsr1p Antibody (31C4) [NBP1-05389] - Western blot analysis of Nsr1p expression in homogenates of yeast extracts.

Product Discontinued

Nsr1p Antibody (31C4) Summary

• Description: 31C4 was isolated during a screen for monoclonal antibodies reactive with yeast nucleolar proteins. We determined the specificity by screening a yeast genomic library expressed as b-galactosidase fusion proteins in lgt11. Two individually isolated, different positive clones were found. Sequencing revealed that in both clones b-galactosidase was fused at the EcoRI site to the amino acid sequence beginning with EFEH, corresponding to amino acids 190 and onwards in Nsr1p. This peptide is ~20 amino acids into the first RRM domain. Thus, reacts with an epitope in the C-terminal 55% of Nsr1p. The antibody is provided as sterile-filtered cell culture fluid from an Integra CL-350 biochamber plus sodium azide. The IgG isotype and exact concentration are not known.
• Immunogen: Yeast nuclear preparation.
• Marker: Nucleolar Marker
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:500
  • Western Blot 1:10000
• Application Notes: This Nsr1p (31C4) antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot, where a band can be seen at ~44.5 kDa.
• Theoretical MW: 44.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

S. cerevisiae

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Supplied as concentrated hybridoma cell culture media.
• Preservative: 0.035% Sodium Azide
• Purity: Tissue culture supernatant

Alternate Names for Nsr1p Antibody (31C4)

• NSR 1
• Nsr 1p
• NSR1
• Nuclear localization sequence binding protein
• p67
• SHE 5
• SHE5

Background

Yeast protein Nsr1p is identical to well known protein nucleolin functionally as well as in sequence homology: Nsr1p has an acidic N-terminal region with several putative phosphorylation sites, a middle region with two RBDs, and a C-terminal GAR domain. Nsr1p physically interacts with yeast enzyme topoisomerase I, yTop1p and has been shown to be implicated in cellular localization of Top1 and that this localization could be central in determining sensitivity to drugs that target topoisomerases. Nucleolin/Nsr1p is a ubiquitously expressed protein localized to nucleolus and is implicated in ribosome biogenesis, , transcription of rDNA, processing as well as modification of rRNA, nuclear- cytosolic transport of ribosomal protein and ribosomal subunits by shuttling between the nucleus and the cytoplasm.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.