![Immunohistochemistry-Paraffin: ALK/CD246 Antibody (5A4) [NB120-17127] - Formalin fixed paraffin embedded anaplastic lymphoma kinase stained with ALK/CD246 antibody (NB120-17127).](http://images.novusbio.com/fullsize/ALK-CD246-Antibody-5A4-Immunohistochemistry-Paraffin-NB120-17127-img0002.jpg "Immunohistochemistry-Paraffin: ALK/CD246 Antibody (5A4) [NB120-17127] - Formalin fixed paraffin embedded anaplastic lymphoma kinase stained with ALK/CD246 antibody (NB120-17127).")
| Reactivity | HuSpecies Glossary |
| Applications | IHC |
| Clone | 5A4 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | Prokaryotic recombinant protein corresponding to a region which spans the tyrosine kinase catalytic domain and part of the C-terminus of NPM-ALK transcript (419-520 aa). |
| Localization | Cell Membrane |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Tissue culture supernatant |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | IHC-P: recommended pretreatment of citrate buffer, pH 6.0. Recommended incubation time of 30 min at RT. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | Tissue culture supernatant |
| Preservative | 0.05% Sodium Azide |
| Purity | Tissue culture supernatant |
Secondary Antibodies |
Isotype Controls |
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![Immunohistochemistry-Paraffin: ALK/CD246 Antibody (5A4) [NB120-17127] - Formalin-fixed, paraffin-embedded human anaplastic lymphoma stained with p80/ALK using peroxidase-conjugate and AEC chromogen. Note nuclear and cytoplasmic staining of tumor cells.](http://images.novusbio.com/fullsize/ALK-CD246-Antibody-5A4-Immunohistochemistry-Paraffin-NB120-17127-img0001.jpg "Immunohistochemistry-Paraffin: ALK/CD246 Antibody (5A4) [NB120-17127] - Formalin-fixed, paraffin-embedded human anaplastic lymphoma stained with p80/ALK using peroxidase-conjugate and AEC chromogen. Note nuclear and cytoplasmic staining of tumor cells.")
![Immunohistochemistry-Paraffin: Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] [NB7539] - FFPE serial sections of human stomach carcinoma. Primary Antibody: Mouse anti-p53 (Clone DO-1) used at a dilution of 1:100. Secondary Antibody: Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] Conjugated used at a dilution of 1:200 (5ug/ml). Detection: DAB](https://images.novusbio.com/images/Goat-anti-Mouse-IgG-H+L-Secondary-Antibody-HRP-Immunohistochemistry-Paraffin-NB7539-img0014.jpg "Immunohistochemistry-Paraffin: Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] [NB7539] - FFPE serial sections of human stomach carcinoma. Primary Antibody: Mouse anti-p53 (Clone DO-1) used at a dilution of 1:100. Secondary Antibody: Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] Conjugated used at a dilution of 1:200 (5ug/ml). Detection: DAB")
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")