![ELISA: Norovirus VP1 Antibody (A1431) - Chimeric - BSA Free [NBP3-43556] - Binding curves of NBP3-43556 (blue) and an isotype control (anti-fluorescein; orange) on an ELISA plate coated with Norovirus VP1 at 2.5 µg/mL. A 3-fold serial dilution from 3,000 ng/mL to 0.0169 ng/mL was assessed. Detection was performed with an HRP-conjugated anti-rabbit IgG antibody (Goat Anti-Rabbit IgG, Fc-fragment specific, minimum cross-reactivity to human serum proteins) at 0.25 µg/mL.](http://images.novusbio.com/fullsize/nbp3-43556_mouse-norovirus-vp1-mab-a1431-chimeric-azide-bsa-free-elisa-222026032208.png "ELISA: Norovirus VP1 Antibody (A1431) - Chimeric - BSA Free [NBP3-43556] - Binding curves of NBP3-43556 (blue) and an isotype control (anti-fluorescein; orange) on an ELISA plate coated with Norovirus VP1 at 2.5 µg/mL. A 3-fold serial dilution from 3,000 ng/mL to 0.0169 ng/mL was assessed. Detection was performed with an HRP-conjugated anti-rabbit IgG antibody (Goat Anti-Rabbit IgG, Fc-fragment specific, minimum cross-reactivity to human serum proteins) at 0.25 µg/mL.")
| Reactivity | V-ViSpecies Glossary |
| Applications | ELISA, B/N |
| Clone | A1431 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Description | This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG1 format for improved compatibility with existing reagents assays and techniques. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | The original antibody was isolated from an anti-NoV GII.4c IgG serum antibody repertoire generated from three donors that experienced a significant increase in GII.4 titer after immunization with the bivalent GII.4c + GI.1 VLP vaccine. (Uniprot# S5YAA0) |
| Specificity | This antibody recognizes conserved residues distal to the HBGA binding pocket but accessible on the assembled VLP. It binds each P domain monomer at a cleft between the P1 and P2 subdomains. Norovirus (NoVs) is one of the main pathogens causing sporadic cases and large outbreaks of acute gastroenteritis, and norovirus can infect people of all ages. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Proclin 300 |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
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![Immunohistochemistry-Paraffin: Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] [NB7539] - FFPE serial sections of human stomach carcinoma. Primary Antibody: Mouse anti-p53 (Clone DO-1) used at a dilution of 1:100. Secondary Antibody: Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] Conjugated used at a dilution of 1:200 (5ug/ml). Detection: DAB](https://images.novusbio.com/images/Goat-anti-Mouse-IgG-H+L-Secondary-Antibody-HRP-Immunohistochemistry-Paraffin-NB7539-img0014.jpg "Immunohistochemistry-Paraffin: Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] [NB7539] - FFPE serial sections of human stomach carcinoma. Primary Antibody: Mouse anti-p53 (Clone DO-1) used at a dilution of 1:100. Secondary Antibody: Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] Conjugated used at a dilution of 1:200 (5ug/ml). Detection: DAB")
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")