![ELISA: Norovirus VP1 Antibody (A1227) - Chimeric - BSA Free [NBP3-43558] - Binding curves of Norovirus VP1 (red) and an isotype control (anti-fluorescein; orange) on an ELISA plate coated with Norovirus VP1 at 2.5 µg/mL. A 3-fold serial dilution from 3,000 ng/mL to 0.0169 ng/mL was assessed. Detection was performed with an HRP-conjugated anti-rabbit IgG antibody (Goat Anti-Rabbit IgG, Fc-fragment specific, minimum cross-reactivity to human serum proteins) at 0.25 µg/mL.](http://images.novusbio.com/fullsize/nbp3-43558_mouse-norovirus-vp1-mab-a1227-chimeric-azide-bsa-free-elisa-2220260351114.png "ELISA: Norovirus VP1 Antibody (A1227) - Chimeric - BSA Free [NBP3-43558] - Binding curves of Norovirus VP1 (red) and an isotype control (anti-fluorescein; orange) on an ELISA plate coated with Norovirus VP1 at 2.5 µg/mL. A 3-fold serial dilution from 3,000 ng/mL to 0.0169 ng/mL was assessed. Detection was performed with an HRP-conjugated anti-rabbit IgG antibody (Goat Anti-Rabbit IgG, Fc-fragment specific, minimum cross-reactivity to human serum proteins) at 0.25 µg/mL.")
| Reactivity | V-ViSpecies Glossary |
| Applications | ELISA |
| Clone | A1227 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Description | This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG1 format for improved compatibility with existing reagents assays and techniques. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | The original antibody was isolated from an anti-NoV GII.4c IgG serum antibody repertoire generated from three donors that experienced a significant increase in GII.4 titer after immunization with the bivalent GII.4c + GI.1 VLP vaccine. (Uniprot# Q83883) |
| Specificity | This antibody shows broadly binding but no blocking activity toward multiple GI and GII strains such as GI.I, GII.2, and GII.17. This antibody recognizes a highly conserved epitope located at an occluded site on the norovirus capsid, at the interface between the P domain and shell domain of the Norovirus, which explain its high level of cross-reactivity. Norovirus (NoVs) is one of the main pathogens causing sporadic cases and large outbreaks of acute gastroenteritis, and norovirus can infect people of all ages. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Proclin 300 |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
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![Immunohistochemistry-Paraffin: Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] [NB7539] - FFPE serial sections of human stomach carcinoma. Primary Antibody: Mouse anti-p53 (Clone DO-1) used at a dilution of 1:100. Secondary Antibody: Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] Conjugated used at a dilution of 1:200 (5ug/ml). Detection: DAB](https://images.novusbio.com/images/Goat-anti-Mouse-IgG-H+L-Secondary-Antibody-HRP-Immunohistochemistry-Paraffin-NB7539-img0014.jpg "Immunohistochemistry-Paraffin: Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] [NB7539] - FFPE serial sections of human stomach carcinoma. Primary Antibody: Mouse anti-p53 (Clone DO-1) used at a dilution of 1:100. Secondary Antibody: Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] Conjugated used at a dilution of 1:200 (5ug/ml). Detection: DAB")
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")