NM23-H1/H2 Antibody

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, A431 human epithelial carcinoma cell line, HT-2 mouse T cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF Membrane was probed with 0.2 µg/mL of Human/Mouse/Rat NM23-H1/H2 Polyclonal Antibody (Catalog # AF6019) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for NM23-H1/H2 at approximately 17-20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

NM23-H1/H2 was detected in immersion fixed MCF-7 human breast cancer cell line using Sheep Anti-Human/Mouse/Rat NM23-H1/H2 Polyclonal Antibody (Catalog # AF6019) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

NM23-H1/H2 Antibody Summary

• Immunogen: E. coli-derived recombinant human NM23‑H1
  Met1-Glu152
  Accession # P15531
• Specificity: Detects human, mouse, and rat NM23-H1/H2 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: NME1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NM23-H1/H2 Antibody

• AL024257;AWD;GAAD;NB;NBS;NDKA;NDKB;NDPKA;NDPK-A;NDPKB;NDPK-B;NM23;nm23-2;NM23A;NM23B;NM23-H1;NM23-H2;NM23-M1;nm23-M2;NME1;NME2;p18-12d;PUF
• NM23-H1/H2
• NM23H2

Background

NM23-H1 (Non-metastatic protein 23 homolog 1; also NDKA) is a 17-20 kDa member of the NDK family of enzymes. NM23-H1 is ubiquitous in expression, and performs multiple functions. It forms disulfide-linked homohexamers, and heterohexamers with NM23-H2, generating a nucleoside diphosphate kinase that catalyzes a phosphoryl transfer from ATP to a nucleoside diphosphate. It also shows histidine protein and Ser/Thr protein kinase activity, and forms covalent linkages with molecules diverse as p53 and STRAP. It is found both intracellularly, and in blood at ng/mL concentrations. Human NM23-H1 is 152 amino acids (aa) in length, contains one NDP kinase domain (aa 5-134) and shows acetylation at Ala2 and Lys56, plus phosphorylation at Tyr52, Thr94, Ser122 and Ser125. Human NM23-H1 shares 89% aa identity with human 17-18 kDa NM23-H2 (Non-metastatic protein 23 homolog 2; also NDKB), and 94% aa identity with mouse NM23-H1. A second H1 isoform named NM23-H1B with 25 additional aa at the N-terminus has also been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NME1
• Uniprot:
  • P15531()