Nitrotyrosine Antibody (HM11) [Biotin]

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Immunohistochemistry-Paraffin: Nitrotyrosine Antibody (HM11) [Biotin] [NB110-2023] - Nitrotyrosine in human liver of severely obesed patients. Staining of paraffin tissue section with clone HM.11. Anti-nitrotyrosine ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-Fr, IHC-P
Clone
HM11
Clonality
Monoclonal
Host
Mouse
Conjugate
Biotin

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Catalog# & Conjugate Size Price

Nitrotyrosine Antibody (HM11) [Biotin] Summary

Immunogen
Nitrated KLH
Specificity
This highly specific monoclonal antibody HM.11 reacts with nitrotyrosine, both with the free amino acid as well as with proteins containing nitrotyrosine. The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic plaques. Nitrotyrosine is formed in tissues in presence of the active metabolite NO and is a stable end product of nitrosylation of tyrosine. Various pathways including the formation of peroxinitrite lead to nitrotyrosine production.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Readout System

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
10 mM Sodium Phophsate (pH 7.6), 0.25 M NaCl, and 15 mg/ml BSA
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Alternate Names for Nitrotyrosine Antibody (HM11) [Biotin]

  • Nitrotyrosine

Background

The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic placques. Nitrotyrosine is formed in tissues in presence of the active metabolite NO. Various pathways including the formation of peroxinitrite lead to nitrotyrosine production.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional Nitrotyrosine Products

Nitrotyrosine NB110-2023

Research Areas for Nitrotyrosine Antibody (NB110-2023)

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