Nitrotyrosine Antibody (HM.11) [Biotin]

Immunohistochemistry-Paraffin: Nitrotyrosine Antibody (HM11) [Biotin] [NB110-2023] - Nitrotyrosine in human liver of severely obesed patients. Staining of paraffin tissue section with clone HM.11. Anti-nitrotyrosine at 2 ug/ml (1h, RT).

Product Discontinued

Nitrotyrosine Antibody (HM.11) [Biotin] Summary

• Immunogen: Nitrated KLH
• Specificity: This highly specific monoclonal antibody HM.11 reacts with nitrotyrosine, both with the free amino acid as well as with proteins containing nitrotyrosine. The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic plaques. Nitrotyrosine is formed in tissues in presence of the active metabolite NO and is a stable end product of nitrosylation of tyrosine. Various pathways including the formation of peroxinitrite lead to nitrotyrosine production.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:100-1:2000
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 10 mM Sodium Phophsate (pH 7.6), 0.25 M NaCl, and 15 mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for Nitrotyrosine Antibody (HM.11) [Biotin]

• Nitrotyrosine

Background

The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic placques. Nitrotyrosine is formed in tissues in presence of the active metabolite NO. Various pathways including the formation of peroxinitrite lead to nitrotyrosine production.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.