![Immunohistochemistry-Frozen: Nitrotyrosine Antibody (EM-30) [NBP1-96130] - Human tumoral colon epithelium with anti-nitrotyrosine antibody (EM-30).](http://images.novusbio.com/fullsize/Nitrotyrosine-Antibody-EM-30-Immunohistochemistry-Frozen-NBP1-96130-img0001.jpg "Immunohistochemistry-Frozen: Nitrotyrosine Antibody (EM-30) [NBP1-96130] - Human tumoral colon epithelium with anti-nitrotyrosine antibody (EM-30).")
| Reactivity | Mu, All-NASpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | EM-30 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Description | Novus Biologicals Mouse Nitrotyrosine Antibody (EM-30) - BSA Free (NBP1-96130) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Nitrotyrosine Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | NO2-Tyr-CH2-Thyroglobulin |
| Specificity | The antibody EM-30 detects nitrotyrosine group in biological material. It can be used as a marker for peroxynitrite formation in particular tissues |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | This antibody I useful for Western Blot (Positive control:NO2-BSA, NO2-HS) Immunohistochemistry-paraffin( Positive tissue: colon cancer epithelium, prostate hyperplasia)and Immunohistochemistry-frozen (Positive tissue: colon cancer epithelium, prostate hyperplasia) Use in ICC/IF reported in secitific publication PMID: 32793221 |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Phosphate buffered saline (PBS), pH 7.4 |
| Preservative | 15mM Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
| Publication using NBP1-96130 | Applications | Species |
|---|---|---|
| Soto M, Delatorre N, Hurst C, Rodgers K Targeting the Protective Arm of the Renin-Angiotensin System to Reduce Systemic Lupus Erythematosus Related Pathologies in MRL-lpr Mice Front. Immunol. 2020-07-23 [PMID: 32793221] (ICC/IF, Mouse) | ICC/IF | Mouse |
Secondary Antibodies |
Isotype Controls |
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")