Neuraminidase Antibody

• Reactivity: V
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Neuraminidase Antibody Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant Influenza A Virus H1N1 Neuraminidase
  Ser37-Lys469
  Accession # AAF77036
• Specificity: Detects Influenza A Virus H1N1 Neuraminidase in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity: Immunogen affinity purified
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.1 ug/mL

Reactivity Notes

Influenza A Virus H1

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Neuraminidase Antibody

• NANH
• Sialidase
• Viral Neuraminidase

Background

Neuraminidase (NA) and hemagglutinin (HA) are major membrane glycoproteins found on the surface of influenza virus. HA is a lectin that binds sialic acid on host cell membrane. NA is a sialic acid hydrolase that specifically clips off terminally located sialic acid on host cell surface. The two proteins are essential for the infectious cycle of the influenza virus. During initial infection, an influenza virus will hold onto an epithelial cell through HA-sialic acid interaction. At the end of an infectious cycle, the NA will cleave the sialic acid on the host cell membrane, releasing the formed viral particle from the HA-sialic acid bondage (1). The neuraminidase activity is also thought to help the virus penetrate mucus. Nine subtypes of NA have been identified, all of which are tetrameric and share a common structure consisting of a globular head, a thin stalk region, and a small hydrophobic region that anchors the protein in the virus membrane (2). The purified recombinant viral H1N1NA consists of amino acid residues 37 to 469 as deduced from the 1918 Spanish flu virus NA (A/Bervig_Mission/1/18) (3). It has a distinct N-glycan profile and is resistant to trypsin digestion (4).

1. Palese, P. & Compans, R. W. (1976) J. Gen. Virol. 33:159.
2. Colman, P. M. et al. (1983) Nature 303:41.
3. Reid, A. (2000) Proc. Natl. Acad. Sci. USA 97:6785.
4. Wu, Z.L. et al. (2009) Biochem. Biophys. Res. Comm. 379:749.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Neuraminidase Antibody (AF4858)(2)

Publications using AF4858

• M Kawahara, T Wada, F Momose, E Nobusawa, Y Morikawa Cell-Based Influenza A/H1N1pdm09 Vaccine Viruses Containing Chimeric Hemagglutinin with Improved Membrane Fusion Ability Vaccines (Basel), 2020;8(3):. 2020 [PMID: 32825107] (Western Blot, Human)
• Influenza A Virus Hemagglutinin is Required for the Assembly of Viral Components Including Bundled vRNPs at the Lipid Raft Viruses, 2016;8(9):. 2016 [PMID: 27626438]

Bioinformatics

• Uniprot:
  • AAF77036(Viral)