NCKAP5L Antibody

Western Blot: NCKAP5L Antibody [NBP2-78830] - Detection of human and mouse NCKAP5L by western blot. Samples: Whole cell lysate (10 ug) from K-562, Hep-G2, LNCaP, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NCKAP5L antibody NBP2-78830 used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunoprecipitation: NCKAP5L Antibody [NBP2-78830] - Detection of human NCKAP5L by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from K-562 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NCKAP5L antibody NBP2-78830 used for IP at 100 ul per reaction. NCKAP5L was also immunoprecipitated by another rabbit anti-NCKAP5L antibody. For blotting immunoprecipitated NCKAP5L, NBP2-78830 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.

Product Discontinued

NCKAP5L Antibody Summary

• Immunogen: The epitope recognized by A305-888A-M maps to a region between residue 1025 and 1075 of human Nck-associated protein 5-like using the numbering given in entry Q9HCH0.2 (GeneID 57701).
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NCKAP5L
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 50-100 ul/mg lysate
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at 2 - 8 C / 1 year from date of receipt
• Buffer: Tris-buffered Saline, 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.04 mg/ml
• Purity: Affinity purified

Alternate Names for NCKAP5L Antibody

• FLJ36581
• KIAA1602
• NCK-associated protein 5-like

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NCKAP5L