Native Lipoteichoic Acid Antibody (55) - BSA Free

• Reactivity: Ba
• Applications: WB, ELISA, Flow, Func, ICC/IF, IHC
• Clone: 55
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Native Lipoteichoic Acid Antibody (55) - BSA Free Summary

• Description: Please See references for verifying information below:
  IA: For detection, 1.2 ug/ml antibody in PBS was added for 1 hr at 37 C on LTA coated plates
  FC: 1/250
  IF: 60' in PBS/0.02%BSA/0.02% saponin
  W: A reduced or native sample treatment and run on 15% SDS-Page. Blot was incubated o/n at 4C with a 1/1000 dilution .The band size is ~17 kDa
• Immunogen: Microbial mixture of Streptococcus sobrims HG961, HG962, HG970, and HG977
• Isotype: IgG3
• Clonality: Monoclonal
• Host: Mouse
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:250
  • Functional
  • Immunoassay 1.2 ug/ml in PBS
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Western Blot 1:1000

Reactivity Notes

The specificity is labled not applicable. Bacteria reactivity reported in scientific literature (PMID:33055259).

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: Culture medium + 0.02% NaN3
• Preservative: 0.02% Sodium Azide
• Purity: Tissue culture supernatant

Alternate Names for Native Lipoteichoic Acid Antibody (55) - BSA Free

• LTA

Background

The monoclonal antibody 55 recognizes lipoteichoic acid (LTA). LTA, a glycerol phosphate surface polymer, is a component of the envelope of Gram-positive bacteria. LTA is anchored via its glycolipids to the membrane and carries a polysaccharide chain extending into the peptidoglycan layer of the cell wall. LTA is released spontaneously into the culture medium during growth of gram-positive bacteria. LTA functions as an immune activator with characteristics very similar to lipopolysaccharide (LPS) from Gram-negative bacteria. LTA binds to CD14 and triggers activation predominantly via Toll-like receptor 2. Although LTA is internalized and traffics to the Golgi, the cellular activation in response to LTA occurs at the cell surface.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Native Lipoteichoic Acid Antibody (NBP1-60146)(11)

Publications using NBP1-60146

• Hirose Y, Murosaki S, Fujiki T et al. Lipoteichoic acids on Lactobacillus plantarum cell surfaces correlate with induction of interleukin-12p40 production. Microbiol Immunol. 2010-03-01 [PMID: 20236424]
• Jimenez-Dalmaroni MJ, Xiao N, Corper AL et al. Soluble CD36 ectodomain binds negatively charged diacylglycerol ligands and acts as a co-receptor for TLR2. PLoS One. 2009-10-01 [PMID: 19847289]
• Yajima A, Takahashi Y, Shimazu K et al. Contribution of phosphoglucosamine mutase to the resistance of Streptococcus gordonii DL1 to polymorphonuclear leukocyte killing. FEMS Microbiol Lett. 2009-08-01 [PMID: 19552711]
• Grundling A, Schneewind O. Synthesis of glycerol phosphate lipoteichoic acid in Staphylococcus aureus. Proc Natl Acad Sci U S A. 2007-05-01 [PMID: 17483484]
• Henneke P, Morath S, Uematsu S et al. Role of lipoteichoic acid in the phagocyte response to group B streptococcus. J Immunol. 2005-05-01 [PMID: 15879147]
• Triantafilou M, Manukyan M, Mackie A et al. Lipoteichoic acid and toll-like receptor 2 internalization and targeting to the Golgi are lipid raft-dependent. J Biol Chem. 2004-09-01 [PMID: 15247273]
• van Langevelde P, Ravensbergen E, Grashoff P et al. Antibiotic-induced cell wall fragments of Staphylococcus aureus increase endothelial chemokine secretion and adhesiveness for granulocytes. Antimicrob Agents Chemother. 1999-12-01 [PMID: 10582893]
• van Langevelde P, van Dissel JT, Ravensbergen E et al. Antibiotic-induced release of lipoteichoic acid and peptidoglycan from Staphylococcus aureus: quantitative measurements and biological reactivities. Antimicrob Agents Chemother. 1998-12-01 [PMID: 9835493]
• Hogg SD, Whiley RA, De Soet JJ et al. Occurrence of lipoteichoic acid in oral streptococci. Int J Syst Bacteriol. 1997-01-01 [PMID: 8995803]
• Qin X, Zhu F, Li W et al. iTie controls intestinal tolerance through suppressing the NLRP6 inflammasome bioRxiv 2023-01-12
• Chen YM, Tsai PH, Ye ZS et al. Functional Analysis of the Collagen Binding Proteins of Streptococcus parasanguinis FW213 mSphere 2020-10-14 [PMID: 33055259] (Bacteria)