Mycobacterium Tuberculosis CFP10 Antibody (KFB16) [Alexa Fluor® 700]

• Reactivity: Ba
• Applications: DB, ELISA
• Clone: KFB16
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 700

Mycobacterium Tuberculosis CFP10 Antibody (KFB16) [Alexa Fluor® 700] Summary

• Immunogen: Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with recombinant protein CFP10 M. tuberculosis H37RV.
• Specificity: CFP antigen.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Dot Blot
  • ELISA
  • Sandwich ELISA
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Bacteria

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Mycobacterium Tuberculosis CFP10 Antibody (KFB16) [Alexa Fluor® 700]

• 10 kDa culture filtrate antigen cfp10
• Culture filtrate protein 10
• ESAT 6 like protein esxB
• esxB
• lhp
• mtsA10
• Secreted antigenic protein MTSA 10
• tuberculosis CFP10

Background

CFP10 (10 kDa culture filtrate protein) is a low molecular weight protein isolated from Mycobacterium tuberculosis culture filtrates. CFP10 and the 6 kDa early secretory antigen of T cells (ESAT6) are secreted in abundance by Mycobacterium tuberculosis and are frequently recognized by T cells from infected people. The genes encoding these proteins have been deleted from the genome of the vaccine strain Bacille Calmette Guerin (BCG). CFP10 is one of a small number of secreted M. tuberculosis proteins now identified, that appear to play key roles in tuberculosis pathogenesis and in the stimulation of immunity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.