Mycobacterium avium subsp. paratuberculosis Antibody (15D10)

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Competitive ELISA: Mycobacterium avium subsp. paratuberculosis Antibody (15D10) [NBP2-54632] - Bacterial suspension of irradiated Mycobacterium avium subsp. paratuberculosis (MAP) was added to wells as "free" MAP cells ...read more

Product Details

Summary
Reactivity BaSpecies Glossary
Applications ELISA, IP, ELISA, ELISA(Comp)
Clone
15D10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Mycobacterium avium subsp. paratuberculosis Antibody (15D10) Summary

Immunogen
Ethanol-extracted surface antigens of gamma-irradiated MAP strain B4 cells
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Purity
Thiophilic Chromatography
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Competitive ELISA
  • Direct ELISA
  • ELISA 1:100 - 1:2000
  • Immunoprecipitation 1:10- 1:500
Application Notes
Direct and Competitive ELISA: A study evaluating the specificity of immunocapture (PMID: 26815790) assayed three non-target Mycobacterium spp. (M. avium subsp. avium, M. bovis BCG and M. terrae). Beads coated with 6G11, the bead combination MAb 6G11 and 15D10 (50:50 mix), and dually-coated MAb 6G11 and 54.3.1AA beads demonstrated a low percentage of non-specific capture (ranging from 0.75-15% capture, with M. bovis BCG giving the highest percentage capture for all bead types).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Thiophilic Chromatography

Alternate Names for Mycobacterium avium subsp. paratuberculosis Antibody (15D10)

  • Mycobacterium paratuberculosis

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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