![Western Blot: Myc Epitope Tag Antibody (2) [NBP2-43627] - Myc tag antibody [0002] Immunoprecipitation of Myc tagged protein. HeLa transfected lysates were subjected to immunoprecipitation using (B) normal mouse IgG or (C) 2.5 ug of anti-Myc tag antibody [0002] . (A) Input, 20ug of HeLa transfected lysates. The precipitated protein was detected. EasyBlot anti-Mouse IgG Kit was used in Western blot.](http://images.novusbio.com/fullsize/Myc-Epitope-Tag-Antibody-2-Western-Blot-NBP2-43627-img0004.jpg "Western Blot: Myc Epitope Tag Antibody (2) [NBP2-43627] - Myc tag antibody [0002] Immunoprecipitation of Myc tagged protein. HeLa transfected lysates were subjected to immunoprecipitation using (B) normal mouse IgG or (C) 2.5 ug of anti-Myc tag antibody [0002] . (A) Input, 20ug of HeLa transfected lysates. The precipitated protein was detected. EasyBlot anti-Mouse IgG Kit was used in Western blot.")
| Reactivity | ETSpecies Glossary |
| Applications | WB, ICC/IF, IP |
| Clone | 2 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Description | Novus Biologicals Mouse Myc Epitope Tag Antibody (2) - Azide and BSA Free (NBP2-43627) is a monoclonal antibody validated for use in WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | The immunogen used to generate this antibody corresponds to Myc Epitope Tag |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: Myc Epitope Tag Antibody (2) [NBP2-43627] - Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Myc tag stained by Myc tag antibody [0002] (NBP2-43627) diluted at 1:2000.](http://images.novusbio.com/fullsize/Myc-Epitope-Tag-Antibody-2-Immunocytochemistry-Immunofluorescence-NBP2-43627-img0006.jpg "Immunocytochemistry/Immunofluorescence: Myc Epitope Tag Antibody (2) [NBP2-43627] - Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Myc tag stained by Myc tag antibody [0002] (NBP2-43627) diluted at 1:2000.")
![Western Blot: Myc Epitope Tag Antibody (2) [NBP2-43627] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Myc Epitope Tag antibody [GT0002] (NBP2-43627) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43627_mouse-monoclonal-myc-epitope-tag-antibody-2-910202420104447.jpg "Western Blot: Myc Epitope Tag Antibody (2) [NBP2-43627] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Myc Epitope Tag antibody [GT0002] (NBP2-43627) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Indirect ELISA analysis was performed by coating the plate with recombinant Multiple Tags Positive Control ( 6xHis-MBP-DDDDK-V5-HSV-HA-T7-Myc-S) (57.14-0.89 nM). Coated protein was probed with Myc tag antibody [GT0002] (NBP2-43627) (1 ug/mL). Goat antibody Mouse IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.](http://images.novusbio.com/fullsize/nbp2-43627_mouse-monoclonal-myc-epitope-tag-antibody-2-elisa-2611202518395724.jpg "Indirect ELISA analysis was performed by coating the plate with recombinant Multiple Tags Positive Control ( 6xHis-MBP-DDDDK-V5-HSV-HA-T7-Myc-S) (57.14-0.89 nM). Coated protein was probed with Myc tag antibody [GT0002] (NBP2-43627) (1 ug/mL). Goat antibody Mouse IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.")
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")