MSK1/MSK2 [p Ser376, p Ser360] Antibody [Unconjugated]

Western blot shows lysates of HEK293 human embryonic kidney cell line untreated (-) or treated (+) with 200 ng/mL Recombinant Human EGF (Catalog # 236-EG) in the presence or absence of pathway inhibitors SB203580 and PD98059 for 20 minutes. PVDF membrane was probed with 0.2 µg/mL of Rabbit Anti-Human Phospho-MSK1/MSK2 (MSK1 S376, MSK2 S360) Antigen Affinity-purified Polyclonal Antibody, followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-MSK1/MSK2 (MSK1 S376, MSK2 S360) at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: LYOPH

MSK1/MSK2 [p Ser376, p Ser360] Antibody [Unconjugated] Summary

• Description: Antibody detects MSK1 [p Ser376] and MSK2 [p Ser360]
• Immunogen: Phosphopeptide containing human MSK1 S376 site
• Modification: p Ser376, p Ser360
• Specificity: Detects human MSK1 and MSK2 when phosphorylated at S376 and S360, respectively. The amino acid sequence surrounding S360 of mouse MSK2 is identical to the phosphopeptide antigen.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MSK1/MSK2 [p Ser376, p Ser360] Antibody [Unconjugated]

• 1110069D02Rik
• 3110005L17Rik
• 6330404E13Rik
• MSK1/MSK2

Background

Mitogen- and stress-activated protein kinase 1 (MSK1), also known as ribosomal protein S6 kinase 5 (RPS6KA5), belongs to the AGC family of kinases. Both MSK1 and the related MSK2, also known as RSKB and RPS6KA4, have two kinase domains connected by a regulatory linker region, and are activated by the mitogen-activated protein kinases ERK1, ERK2, and p38. Nuclear MSK phosphorylates and activates a number of transcription factors, including ATF1 and CREB.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MSK1/MSK2 Antibody (AF1094)(2)

Publications using AF1094

• Z Zhou, S Yoshimatsu, E Qian, M Ishikawa, T Sato, M Ohtaka, M Nakanishi, H Okano Generation of a control human induced pluripotent stem cell line using the defective and persistent Sendai virus vector system Stem Cell Research, 2021-09-23;56(0):102549. 2021-09-23 [PMID: 34598113] (ICC, Human)
• Katarzyna Duda, Lindsey A. Lonowski, Michael Kofoed-Nielsen, Adriana Ibarra, Catherine M. Delay, Qiaohua Kang et al. High-efficiency genome editing via 2A-coupled co-expression of fluorescent proteins and zinc finger nucleases or CRISPR/Cas9 nickase pairs Nucleic Acids Research 2014-06-02 [PMID: 24753413]