![Western Blot: mPlum Antibody [NBP3-11833] - Analysis of mPlum fluorescent protein using rabbit polyclonal antibody PAb (919) on lysates of HEK293T/17 cells co-transfected with mPlum/GPI and YFP/GPI constructs (HEK293T/17 cells transfected with YFP/GPI; negative control) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 ug/ml of rabbit anti-mPlum polyclonal antibody followed by IRDye800-conjugated anti-rabbit secondary antibody. A specific band was detected for mPlum protein at approximately 30 kDa.](http://images.novusbio.com/fullsize/mPlum-Antibody-Western-Blot-NBP3-11833-img0001.jpg "Western Blot: mPlum Antibody [NBP3-11833] - Analysis of mPlum fluorescent protein using rabbit polyclonal antibody PAb (919) on lysates of HEK293T/17 cells co-transfected with mPlum/GPI and YFP/GPI constructs (HEK293T/17 cells transfected with YFP/GPI; negative control) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 ug/ml of rabbit anti-mPlum polyclonal antibody followed by IRDye800-conjugated anti-rabbit secondary antibody. A specific band was detected for mPlum protein at approximately 30 kDa.")
| Reactivity | All-NASpecies Glossary |
| Applications | WB, Flow, ICC/IF |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | mPlum from Discosoma sp. |
| Specificity | The rabbit polyclonal antibody recognizes a far red fluorescent protein tag mPlum. |
| Clonality | Polyclonal |
| Host | Rabbit |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C. Do not freeze. |
| Buffer | Phosphate buffered saline (PBS), pH 7.4 |
| Preservative | 15mM Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Affinity purified |
Secondary Antibodies |
Isotype Controls |
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![Flow Cytometry: mPlum Antibody [NBP3-11833] - Separation of HEK293T/17 cells co-transfected with mPlum/GPI and YFP/GPI constructs stained anti-mPlum Purified rabbit polyclonal antibody (concentration in sample 2 ug/ml, GAR APC, red-filled) from HEK293T/17 cells co-transfected with mPlum/GPI and YFP/GPI constructs unstained by primary polyclonal antibody (GAR APC, black-dashed) in flow cytometry analysis (surface staining) of HEK293T17/mPlum/YFP cell suspension.](http://images.novusbio.com/fullsize/mPlum-Antibody-Flow-Cytometry-NBP3-11833-img0003.jpg "Flow Cytometry: mPlum Antibody [NBP3-11833] - Separation of HEK293T/17 cells co-transfected with mPlum/GPI and YFP/GPI constructs stained anti-mPlum Purified rabbit polyclonal antibody (concentration in sample 2 ug/ml, GAR APC, red-filled) from HEK293T/17 cells co-transfected with mPlum/GPI and YFP/GPI constructs unstained by primary polyclonal antibody (GAR APC, black-dashed) in flow cytometry analysis (surface staining) of HEK293T17/mPlum/YFP cell suspension.")
![Flow Cytometry: mPlum Antibody [NBP3-11833] - Surface staining pattern of HEK293T/17 cells co-transfected with mPlum/GPI and YFP/GPI constructs stained using anti-mPlum Purified rabbit polyclonal antibody (concentration in sample 2 ug/ml, GAR APC).](http://images.novusbio.com/fullsize/mPlum-Antibody-Flow-Cytometry-NBP3-11833-img0002.jpg "Flow Cytometry: mPlum Antibody [NBP3-11833] - Surface staining pattern of HEK293T/17 cells co-transfected with mPlum/GPI and YFP/GPI constructs stained using anti-mPlum Purified rabbit polyclonal antibody (concentration in sample 2 ug/ml, GAR APC).")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")