MIOS Antibody (955413) [Unconjugated]

Western blot shows lysates of MCF-7 human breast cancer cell line, Jurkat human acute T cell leukemia cell line, 3T3-L1 mouse embryonic fibroblast adipose-like cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat MIOS Monoclonal Antibody (Catalog # MAB9289) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MIOS at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 955413
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

MIOS Antibody (955413) [Unconjugated] Summary

• Immunogen: Human MIOS synthetic peptide
  Accession # Q9NXC5
• Specificity: Detects human MIOS in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MIOS Antibody (955413) [Unconjugated]

• FLJ20323
• MIO
• MIOS
• missing oocyte, meiosis regulator, homolog (Drosophila)
• WD repeat-containing protein mio

Background

MIOS is a member of the octameric GATOR (GTPase-activating protein (GAP) activity toward RAGs) complex.  MIOS and fellow GATOR2 subcomplex members WDR24, WDR59, SEH1L and SEC13 inhibit GATOR1 subcomplex members DEPDC5, NPRL2 and NPRL3.  This inhibition by GATOR2 positively regulates mTORC1 signaling, an activity modulated by energy levels, growth factors, and amino acids.  Human MIOS is 875 amino acids (aa) in length, and contains six N-terminal WD40 repeats and a C-terminal zinc finger-like domain.  Over aa 620-875, human MIOS shares 98% identity with mouse and rat MIOS.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MIOS Antibody (MAB9289)(1)

Publication using MAB9289

• Melpomeni Platani, Itaru Samejima, Kumiko Samejima, Masato T. Kanemaki, William C. Earnshaw Seh1 targets GATOR2 and Nup153 to mitotic chromosomes Journal of Cell Science 2018-05-01 [PMID: 29618633]

Bioinformatics

• Uniprot:
  • Q9NXC5()