Recombinant Human Methionine Aminopeptidase 1D/MAP1D Protein

Product Discontinued

Recombinant Human Methionine Aminopeptidase 1D/MAP1D Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 20-335 of Human Methionine Aminopeptidase 1D/MAP1D
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSSSPLNHI YLHKQSSSQQ RRNFFFRRQR DISHSIVLPA AVSSAHPVPK HIKKPDYVTT GIVPDWGDSI EVKNEDQIQG LHQACQLARH VLLLAGKSLK VDMTTEEIDA LVHREIISHN AYPSPLGYGG FPKSVCTSVN NVLCHGIPDS RPLQDGDIIN IDVTVYYNGY HGDTSETFLV GNVDECGKKL VEVARRCRDE AIAACRAGAP FSVIGNTISH ITHQNGFQVC PHFVGHGIGS YFHGHPEIWH HANDSDLPME EGMAFTIEPI ITEGSPEFKV LEDAWTVVSL DNQRSAQFEH TVLITSRGAQ ILTKLPHEA

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: METAP1D
• Purity: >85%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 37.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 0.1 M NaCl, 20% glycerol, 1 mM DTT
• Preservative: No Preservative
• Concentration: 0.25 mg/ml
• Purity: >85%, by SDS-PAGE

Alternate Names for Recombinant Human Methionine Aminopeptidase 1D/MAP1D Protein

• EC 3.4.11.18
• MAP1D
• MAP1DCDS of metAP-3 within PCR fragment
• MetAP1D
• Metap1l
• Methionine Aminopeptidase 1D
• methionine aminopeptidase 1D, mitochondrial
• methionyl aminopeptidase type 1D (mitochondrial)
• Methionyl aminopeptidase type 1D, mitochondrial

Background

Methionine aminopeptidase 1D, mitochondrial precursor, also known as METAP1D, is a 335 amino acid mitochondrial protein that belongs to the peptidase M24A family. It is overexpressed in colon cancer cell lines, suggesting a role in tumorigenesis. METAP1D has also been found to remove methionine from the N-terminus of nascent proteins. METAP1D binds two cobalt ions per subunit and is encoded by a gene that maps to human chromosome 2q31.1. Recombinant human METAP1D protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: METAP1D