Meta-Pneumovirus Antibody (HMPV123) - BSA Free

Immunocytochemistry/Immunofluorescence: Meta-Pneumovirus Antibody (HMPV123) [NBP1-21631] - A Newcastle strain of HMPV grown on LLC MK cells and non infected cells stained with Mouse anti Metapneumovirus, NBP1-21631, and counterstained with Evans Blue.

• Reactivity: V-Vi
• Applications: WB, ICC/IF
• Clone: HMPV123
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Meta-Pneumovirus Antibody (HMPV123) - BSA Free Summary

• Description: Novus Biologicals Mouse Meta-Pneumovirus Antibody (HMPV123) - BSA Free (NBP1-21631) is a monoclonal antibody validated for use in WB, ELISA and ICC/IF. Anti-Meta-Pneumovirus Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Sub-type A hMPV virus isolate NCL03-4/145
• Specificity: NBP1-21631 recognises the nucleoprotein of all sub-types (A1, A2, B1 and B2) of hMPV. NBP1-21631 does not cross-react with cell cultures infected with hRSV, influenzaviruses A and B, adenovirus, parainfluenza viruses 1, 2, 3 and 4b, mumps virus, measles virus, varicella-zoster virus, herpes simplex virus types 1 and 2, human cytomegalovirus, human herpesvirus type 6, ECHOvirus 19, Coxsachievirus B4, poliovirus types 1-3, HHV6 or uninfected HeLa, MA104, 3MK, LLC-MK, HEp2, MRC-5 and HSB-2 cells. It also does not react with sputum bacteria.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: GLYG
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:40-1:80
  • Western Blot 1:200-1:500
• Application Notes: Flow Cytometry reactivty per customer review.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for Meta-Pneumovirus Antibody (HMPV123) - BSA Free

• hMPV
• metapneumovirus

Background

Human metapneumovirus (hMPV) was discovered in 2001, and has been identified worldwide. hMPV is a common respiratory pathogen, particularly in infants and young children. The virus is associated with both upper and lower respiratory tract infections and may be a trigger for asthma. At least two major genotypes of hMPV circulate during community outbreaks.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Meta-Pneumovirus Antibody (NBP1-21631)(3)

Publications using NBP1-21631

• Kumari S, Crim RL, Kulkarni A, Audet SA. Development of a Luciferase Immunoprecipitation Systems Assay to Detect IgG Antibodies Against Human Respiratory Syncytial Virus Nucleoprotein. Clin Vaccine Immunol 2014-01-10 [PMID: 24403526]
• Chang A, Masante C, Buchholz UJ et al. Human metapneumovirus (HMPV) binding and infection are mediated by interactions between the HMPV fusion protein and heparan sulfate. J Virol. 2012-03-01 [PMID: 22238303] (ELISA, Virus)
• Fenwick, F et al. Diagnosis of human metapneumovirus by immunofluorescence staining with monoclonal antibodies in the North-East of England. J Clin Virol 40: 193-196. 2007-01-01 [PMID: 17869169]

Reviews for Meta-Pneumovirus Antibody (NBP1-21631) (4)

Average Rating: 4.3

(Based on 4 reviews)

We have 4 reviews tested in 1 species: Other.

reviewed by: Andres Chang ELISA Other 01/13/2012

Summary

• Application: ELISA
• Sample Tested: HMPV binding on Monkey Kidney cells
• Species: Other

reviewed by: Andres Chang Flow Other 10/13/2010

Summary

• Application: Flow Cytometry
• Sample Tested: Monkey Kidney Fibroblast
• Species: Other

reviewed by: Andres Chang Flow Other 06/30/2010

Summary

• Application: Flow Cytometry
• Sample Tested: Kidney Fibroblast infected with recombinant GFP-expressing HMPV
• Species: Other

reviewed by: Andres Chang IF Other 05/12/2010

Summary

• Application: Immunofluorescence
• Sample Tested: Vero cells
• Species: Other

FAQs for Meta-Pneumovirus Antibody (NBP1-21631). (Showing 1 - 1 of 1 FAQs).

1. What exactly is the nature of the immunogen for this antibody? Is it a purified or isolated protein or a whole virus inoculation? Specifically, I am curious as to whether it is specific for the nucleocapsid protein or some higher order complex.
   • This antibody was generated by immunization with the whole virus. It recognizes the nucleoprotein of all sub-types (A1, A2, B1 and B2) of hMPV.

Bioinformatics

• Gene Symbol: GLYG