Melanoma Associated Antigen (KBA.62) Antibody (KBA.62) - IHC-Prediluted

Immunohistochemistry-Paraffin: Melanoma Associated Antigen (KBA.62) Antibody (KBA.62) - IHC-Prediluted [NBP2-48210] - Formalin-fixed, paraffin-embedded human Tongue stained with Melanoma Monoclonal Antibody (KBA.62).

Immunohistochemistry-Paraffin: Melanoma Associated Antigen (KBA.62) Antibody (KBA.62) - IHC-Prediluted [NBP2-48210] - Formalin-fixed, paraffin-embedded human Melanoma stained with Melanoma Monoclonal Antibody (KBA.62).

• Reactivity: Hu
• Applications: IHC
• Clone: KBA.62
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: IHC-Prediluted

Melanoma Associated Antigen (KBA.62) Antibody (KBA.62) - IHC-Prediluted Summary

• Description: The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.
• Immunogen: Human KAL cells derived from lymph node metastasis of malignant melanoma
• Localization: Cell Surface and Cytoplasmic
• Specificity: KBA.62 is a novel anti-melanoma antibody. It reacts positively against melanocytic tumors but not other tumors, thus demonstrating specificity and sensitivity. Moreover, it reacts positively against junctional nevus cells but not intradermal nevi, and against fetal melanocytes but not normal adult melanocytes. KBA.62 antibody is useful in identifying malignant melanomas. Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, sarcomas, spindle cell carcinomas and various types of mesenchymal neoplasms. A keratin-negative, vimentin-rich neoplasm that immuno-reacts with antibody to S100 protein and with KBA.62 antibody is, with rare exception, a melanoma. Anti-KBA.62 is a useful additional marker for melanoma, specifically in desmoplastic/spindle cell cases and in the context of micro-metastasis in sentinel lymph node.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
• Application Notes: Molecular weight of antigen: (140, 135 and 128kDa and two weak bands of 88 and 73kDa) Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.