Melanoma Associated Antigen (KBA.62) Antibody (KBA.62)

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Immunohistochemistry-Paraffin: Melanoma Associated Antigen (KBA.62) Antibody (KBA.62) [NBP2-45285] - Human Tongue stained with Melanoma Monoclonal Antibody (KBA.62).
Immunohistochemistry-Paraffin: Melanoma Associated Antigen (KBA.62) Antibody (KBA.62) [NBP2-45285] - Human Melanoma stained with Melanoma Monoclonal Antibody (KBA.62).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC, IHC-P, ICC/IF
Clone
KBA.62
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

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Melanoma Associated Antigen (KBA.62) Antibody (KBA.62) Summary

Immunogen
Human KAL cells derived from lymph node metastasis of malignant melanoma
Localization
Cell Surface and Cytoplasmic
Specificity
KBA.62 is a novel anti-melanoma antibody. It reacts positively against melanocytic tumors but not other tumors, thus demonstrating specificity and sensitivity. Moreover, it reacts positively against junctional nevus cells but not intradermal nevi, and against fetal melanocytes but not normal adult melanocytes. KBA.62 antibody is useful in identifying malignant melanomas. Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, sarcomas, spindle cell carcinomas and various types of mesenchymal neoplasms. A keratin-negative, vimentin-rich neoplasm that immuno-reacts with antibody to S-100 protein and with KBA.62 antibody is, with rare exception, a melanoma. Anti-KBA.62 is a useful additional marker for melanoma, specifically in desmoplastic/spindle cell cases and in the context of micro-metastasis in sentinel lymph node.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml
  • Immunofluorescence 0.5 - 1.0 ug/ml
Application Notes
Molecular weight of antigen: (140, 135 and 128kDa and two weak bands of 88 and 73kDa)
Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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