![Immunohistochemistry-Paraffin: Macrophage and Histiocytoma Marker Antibody (D11) - Azide and BSA Free [NBP3-11429] - Human Histiocytoma stained with Macrophage Monoclonal Antibody (D11).](http://images.novusbio.com/fullsize/nbp3-11429_mouse-monoclonal-macrophage-and-histiocytoma-marker-antibody-d11-azide-and-bsa-free-24120258193538.jpg "Immunohistochemistry-Paraffin: Macrophage and Histiocytoma Marker Antibody (D11) - Azide and BSA Free [NBP3-11429] - Human Histiocytoma stained with Macrophage Monoclonal Antibody (D11).")
| Reactivity | Hu, Mu(-), Po(-), Rt(-)Species Glossary |
| Applications | IHC |
| Clone | D11 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-45278). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Membrane preparation from human hepatocytes |
| Localization | Cell Surface & Cytoplasmic |
| Marker | Macrophage & Histiocytoma Marker |
| Specificity | In Western blotting, it detects an antigen of 125kDa in human liver and 135kDa in tumors of histiocytic origin. Comparative study of this monoclonal antibody and a standard CD68 monoclonal antibody showed that their antigens are different. Its antigen in all macrophage types studied is located on the plasma membrane and within cytoplasmic structures including lysosomes. This monoclonal antibody shows a restricted reactivity to cells of the monocyte/macrophage system. It specifically reacts with blood monocytes and stains resident macrophages in a wide variety of human tissues. This monoclonal antibody does not stain antigen-presenting cells, e.g., Langerhans cells. Reportedly, its reactivity is restricted to histiocytes and macrophages. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
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![Immunohistochemistry-Paraffin: Macrophage and Histiocytoma Marker Antibody (D11) - Azide and BSA Free [NBP3-11429] - Human Histiocytoma stained with Macrophage Monoclonal Antibody (D11).](http://images.novusbio.com/fullsize/nbp3-11429_mouse-monoclonal-macrophage-and-histiocytoma-marker-antibody-d11-azide-and-bsa-free-24120258203429.jpg "Immunohistochemistry-Paraffin: Macrophage and Histiocytoma Marker Antibody (D11) - Azide and BSA Free [NBP3-11429] - Human Histiocytoma stained with Macrophage Monoclonal Antibody (D11).")
![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")