Macrophage and Histiocytoma Marker Antibody (D11) - IHC-Prediluted

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Immunohistochemistry-Paraffin: Macrophage and Histiocytoma Marker Antibody (D11) - IHC-Prediluted [NBP2-48138] - Formalin-fixed, paraffin-embedded human Histiocytoma stained with Macrophage Monoclonal Antibody (D11).
Immunohistochemistry-Paraffin: Macrophage and Histiocytoma Marker Antibody (D11) - IHC-Prediluted [NBP2-48138] - Human Histiocytoma stained with Macrophage Monoclonal Antibody (D11).

Product Details

Summary
Reactivity Hu, Mu(-), Po(-), Rt(-)Species Glossary
Applications IHC, IHC-P
Clone
D11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
IHC-Prediluted

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Macrophage and Histiocytoma Marker Antibody (D11) - IHC-Prediluted Summary

Immunogen
Membrane preparation from human hepatocytes
Localization
Cell Surface & Cytoplasmic
Specificity
In Western blotting, it detects an antigen of 125kDa in human liver and 135kDa in tumors of histiocytic origin. Comparative study of this MAb and a standard CD68 MAb showed that their antigens are different. Its antigen in all macrophage types studied is located on the plasma membrane and within cytoplasmic structures including lysosomes. This MAb shows a restricted reactivity to cells of the monocyte/macrophage system. It specifically reacts with blood monocytes and stains resident macrophages in a wide variety of human tissues. This MAb does not stain antigen-presenting cells, e.g., Langerhans cells. Reportedly, its reactivity is restricted to histiocytes and macrophages.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Molecular weight of antigen: 125kDa -135kDa Immunohistochemistry-Paraffin 0.5 - 1 ug/ml for 30 minutes at RT Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.

Reactivity Notes

Does not react with Mouse, Rat or Porcine.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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