Lys20p and Lys21p Antibody (31F5)

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Western Blot: Lys20p and Lys21p Antibody [NBP1-05201] - Western blots of whole yeast protein extracts with a collection of our antibodies. The blot for NBP1-05201 is in the indicated lane, and the number indicates the ...read more

Product Details

Summary
Reactivity YeSpecies Glossary
Applications WB, ICC/IF
Clone
31F5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Lys20p and Lys21p Antibody (31F5) Summary

Immunogen
Lys20p and Lys21p
Specificity
Lys20p and Lys21p
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity
Tissue culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:1000
Application Notes
This Lys20p and Lys21p (31F5) antibody is useful for Western Blot and Immunocytochemistry/Immunofluorescence. In WB bands can be seen at 47.1 kDa (representing Lys20p) and 48.6 kDa (representing Lys21p).

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
47.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Yeast.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
No buffer
Preservative
5mM Sodium Azide
Purity
Tissue culture supernatant

Alternate Names for Lys20p and Lys21p Antibody (31F5)

  • LYS20 Lys20p

Background

In the yeast Saccharomyces cerevisiae, AAA or alpha-amino adipate pathway is responsible for lysine biosynthesis wherein the first committed step involves condensation of acetyl CoA (AcCoA) with alpha-ketoglutarate (alpha-KG) to generate homocitrate as well as CoA in the nucleus, and this reaction is catalyzed by the homocitrate synthase isozymes encoded by LYS20 and LYS21 which belongs to alpha-IPM synthase/homocitrate synthase family and are 92% identical in amino acid sequence. Expression of both LYS20 and LYS21 is upregulated through Gcn4p upon amino acid deprivation, and their positive regulation is also facilitated by Lys14p (transcriptional activator from Zn-Cys cluster family) with co-inducer alpha-AASA (2-aminoadipate semialdehyde). Moreover, LYS20 and LYS21 are positively co-regulated by target of rapamycin or TOR signalling pathway coupled with protein kinase A, through a LYS14-GCN4-independent pathway. Therefore, LYS20 and LYS21 plays a key role in maintaining levels of lysine, an amino acid preferentially located in the vacuole, therefore not available as a source of nitrogen or carbon source, and lysine has been sown to play important physiological roles: contributing to the build-up of H2O2 triggered stress response, prevention of apoptosis induced upon amino acid starvation, and cryoprotection.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Lys20p and Lys21p Antibody (NBP1-05201) (0)

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Secondary Antibodies

 

Isotype Controls

Additional Lys20p and Lys21p Products

Lys20p and Lys21p NBP1-05201

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