Lys20p and Lys21p Antibody (31F5)

Western Blot: Lys20p and Lys21p Antibody [NBP1-05201] - Western blots of whole yeast protein extracts with a collection of our antibodies. The blot for NBP1-05201 is in the indicated lane, and the number indicates the SDS-PAGE molecular weight in kiloDaltons.

• Reactivity: Ye
• Applications: WB, ICC/IF, IHC
• Clone: 31F5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Lys20p and Lys21p Antibody (31F5) Summary

• Description: Novus Biologicals Mouse Lys20p and Lys21p Antibody (31F5) (NBP1-05201) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Yeast nuclear preparation
• Specificity: Lys20p and Lys21p
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:1000-1:10000
  • Immunohistochemistry 1:1000-1:10000
  • Western Blot 1:10000-1:50000
• Application Notes: This Lys20p and Lys21p (31F5) antibody is useful for Western Blot and Immunocytochemistry/Immunofluorescence. In WB bands can be seen at 47.1 kDa (representing Lys20p) and 48.6 kDa (representing Lys21p).
• Theoretical MW: 47.1/48.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Supplied as concentrated hybridoma cell culture media.
• Preservative: 0.035% Sodium Azide
• Purity: Tissue culture supernatant

Alternate Names for Lys20p and Lys21p Antibody (31F5)

• LYS20 Lys20p

Background

In the yeast Saccharomyces cerevisiae, AAA or alpha-amino adipate pathway is responsible for lysine biosynthesis wherein the first committed step involves condensation of acetyl CoA (AcCoA) with alpha-ketoglutarate (alpha-KG) to generate homocitrate as well as CoA in the nucleus, and this reaction is catalyzed by the homocitrate synthase isozymes encoded by LYS20 and LYS21 which belongs to alpha-IPM synthase/homocitrate synthase family and are 92% identical in amino acid sequence. Expression of both LYS20 and LYS21 is upregulated through Gcn4p upon amino acid deprivation, and their positive regulation is also facilitated by Lys14p (transcriptional activator from Zn-Cys cluster family) with co-inducer alpha-AASA (2-aminoadipate semialdehyde). Moreover, LYS20 and LYS21 are positively co-regulated by target of rapamycin or TOR signalling pathway coupled with protein kinase A, through a LYS14-GCN4-independent pathway. Therefore, LYS20 and LYS21 plays a key role in maintaining levels of lysine, an amino acid preferentially located in the vacuole, therefore not available as a source of nitrogen or carbon source, and lysine has been sown to play important physiological roles: contributing to the build-up of H2O2 triggered stress response, prevention of apoptosis induced upon amino acid starvation, and cryoprotection.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.