![Immunocytochemistry/Immunofluorescence: Lymphatic Endothelial Cells Antibody (LA102) - Azide and BSA Free [NB110-96416] - Immunofluorescence staining of mouse Lymphatic Endothelial Cells antigen in primary mouse endothelial cells (mEC) using a monoclonal antibody directed against collagenase- and neuraminidase-treated mouse benign lymphangiomas. As secondary antibody goat anti-rat ALEXA Flour 488 (1:600) was used.](http://images.novusbio.com/fullsize2/Lymphatic-Endothelial-Cells-Antibody-(LA102)---Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NB110-96416-img0015.jpg "Immunocytochemistry/Immunofluorescence: Lymphatic Endothelial Cells Antibody (LA102) - Azide and BSA Free [NB110-96416] - Immunofluorescence staining of mouse Lymphatic Endothelial Cells antigen in primary mouse endothelial cells (mEC) using a monoclonal antibody directed against collagenase- and neuraminidase-treated mouse benign lymphangiomas. As secondary antibody goat anti-rat ALEXA Flour 488 (1:600) was used.")
| Reactivity | MuSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IHC-Fr |
| Clone | LA102 |
| Clonality | Monoclonal |
| Host | Rat |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | LYOPH |
| Immunogen | Collagenase- and neuraminidase-treated mouse benign lymphangiomas |
| Specificity | LA102 reacts with mouse lymphatic vessels except the thoracic duct and the marginal sinus of lymph nodes, but not with any blood vessels. LA102 recognized a protein of around 26 kDa.The antigen recognized by LA102 was localized on both luminal and abluminal lymphatic endothelial cell membranes. Besides lymphatics, some lymphoid cells are also recognized. |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Host | Rat |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | IHC-Fr: acetone fixed or 4% paraformaldehyde in PBS for less than 10 min. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Protein G purified |
| Reconstitution Instructions | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: Lymphatic Endothelial Cells Antibody (LA102) - Azide and BSA Free [NB110-96416] - Lymphatic Endothelial Cells Antibody (LA102) [NB110-96416] - Immunofluorescence staining in primary mouse endothelial cells (mEC) with Lymphatic Endothelial Cells Antibody (LA102) and counter staining of nuclei with Dapi. As secondary antibody goat anti-rat ALEXA Flour 488 was used 1:400.](http://images.novusbio.com/fullsize2/Lymphatic-Endothelial-Cells-Antibody-(LA102)-Immunocytochemistry-Immunofluorescence-NB110-96416-img0013.jpg "Immunocytochemistry/Immunofluorescence: Lymphatic Endothelial Cells Antibody (LA102) - Azide and BSA Free [NB110-96416] - Lymphatic Endothelial Cells Antibody (LA102) [NB110-96416] - Immunofluorescence staining in primary mouse endothelial cells (mEC) with Lymphatic Endothelial Cells Antibody (LA102) and counter staining of nuclei with Dapi. As secondary antibody goat anti-rat ALEXA Flour 488 was used 1:400.")