Ly-6B.2 Antibody (7/4) [Alexa Fluor® 488]

• Reactivity: Mu
• Applications: WB, Flow, ICC/IF, IHC
• Clone: 7/4
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 488

Ly-6B.2 Antibody (7/4) [Alexa Fluor® 488] Summary

• Immunogen: Cultured bone marrow cells
• Specificity: Recognizes Ly6B.2, a polymorphic 40 kDa antigen expressed by polymorphonuclear cells, but absent on resident tissue macrophages. Strains positive for 7/4:129J; AKR; C57BL/6; C57BL/10; C58; DBA/2; NZB; NZW; SJL; MFI; Swiss (PO) Strains Negative/Weak for 7/4:A2G; A/Sn; ASW; BALB/c; C3H/HEH: CBA.T6T6.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Western Blot

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (84%).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ly-6B.2 Antibody (NBP2-13077AF488)(10)

Publications using NBP2-13077AF488

• Sharma S, Wang Q, Muthumalage T, Rahman I. Epithelial Ablation of Miro1/Rhot1 GTPase Augments Lung Inflammation by Cigarette Smoke Pathophysiology 2021-11-26 [PMID: 35366248]
• Qixin Wang, Isaac K. Sundar, Joseph H. Lucas, Thivanka Muthumalage, Irfan Rahman Molecular clock REV-ERB alpha regulates cigarette smoke–induced pulmonary inflammation and epithelial-mesenchymal transition JCI Insight 2021-06-22 [PMID: 34014841]
• Wang Q, Sundar I, Li D et al. E-cigarette-Induced Pulmonary Inflammation and Dysregulated Repair are Mediated by nAChR ?7 Receptor: Role of nAChR ?7 in ACE2 Covid-19 receptor regulation Research Square 2022-11-07 [PMID: 32702718]
• Wang Q, Sundar IK, Li D et al. E-cigarette-induced pulmonary inflammation and dysregulated repair are mediated by nAChR alpha 7 receptor: role of nAChR alpha 7 in SARS-CoV-2 Covid-19 ACE2 receptor regulation Respir. Res. 2020-06-18 [PMID: 32552811] (Mouse)
  
  Details:
  Citation using the Alexa Fluor 488 format of this antibody.
• Wang Q, Khan N A et al Dysregulated repair and inflammatory responses by e-cigarette-derived inhaled nicotine and humectant propylene glycol in a sex-dependent manner in mouse lung FASEB Bioadv 2019-12-12 [PMID: 31825014] (FLOW, FLOW, Mouse)
  
  Details:
  Citation using the Alexa Fluor 488 version of this antibody.
• Rashid K, Sundar IK, Gerloff J et al. Lung cellular senescence is independent of aging in a mouse model of COPD/emphysema. Sci Rep 2018-06-13 [PMID: 29899396] (FLOW, Mouse)
  
  Details:
  This citation used the Alexa Fluor 488 version of this antibody.
• Sundar IK, Rashid K, Gerloff J et al. Genetic ablation of histone deacetylase 2 leads to lung cellular senescence and lymphoid follicle formation in COPD/emphysema. FASEB J 2018-08-09 [PMID: 29630406] (Mouse)
  
  Details:
  This citation used the Alexa Fluor 488 version of this antibody.
• M Evrard, IWH Kwok, SZ Chong, KWW Teng, E Becht, J Chen, JL Sieow, HL Penny, GC Ching, S Devi, JM Adrover, JLY Li, KH Liong, L Tan, Z Poon, S Foo, JW Chua, IH Su, K Balabanian, F Bachelerie, SK Biswas, A Larbi, WYK Hwang, V Madan, HP Koeffler, SC Wong, EW Newell, A Hidalgo, F Ginhoux, LG Ng Developmental Analysis of Bone Marrow Neutrophils Reveals Populations Specialized in Expansion, Trafficking, and Effector Functions Immunity, 2018-02-20;48(2):364-379.e8. 2018-02-20 [PMID: 29466759]
• Sundar IK, Rashid K, Gerloff J, Li D. Genetic Ablation of p16(Ink4a) Does Not Protect Against Cellular Senescence in Mouse Models of COPD/Emphysema. Am. J. Respir. Cell Mol. Biol. 2018-02-15 [PMID: 29447461] (Mouse)
  
  Details:
  This citation used the Alexa Fluor 488 form of this antbody
• Sundar IK, Rashid K, Sellix MT, Rahman I. The nuclear receptor and clock gene REV-ERBa regulates cigarette smoke-induced lung inflammation. Biochem. Biophys. Res. Commun. 2017-12-02 [PMID: 28974420]