LRRC52 Antibody (1037552) [Unconjugated]

HEK293 human embryonic kidney cell line transfected with (A) human LRRC52 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human LRRC52 Monoclonal Antibody (Catalog # MAB10260) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on control antibody staining (MAB002). Staining was performed using our Staining Membrane-associated Proteins protocol.

• Reactivity: Hu
• Applications: Flow
• Clone: 1037552
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

LRRC52 Antibody (1037552) [Unconjugated] Summary

• Immunogen: Human embryonic kidney cell HEK293-derived human LRRC52
  Ser24-Asp244
  Accession # Q8N7C0
• Specificity: Detects human LCCC52 in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LRRC52 Antibody (1037552) [Unconjugated]

• BK Channel Auxiliary Gamma Subunit LRRC52
• FLJ25811
• leucine rich repeat containing 52
• leucine-rich repeat-containing protein 52
• LRRC52

Background

LRRC52 (Leucine-rich repeat-containing protein 52) belongs to an extracellular leucine-rich-repeat–only (Elron) cluster, which includes five other LRRC proteins: LRRC26, LRRC38, LRRC55, LRTM1 and LRTM2 (1). Elron cluster members are type I transmembrane proteins with an extracellular domain (ECD) containing several LRR motifs and a cytoplasmic C-terminal tail with a short stretch of acidic residues (1). The mature ECD of LRRC52 is 221 amino acids (aa), containing 5 LRR motifs, and shares 76% and 78% identity with mouse and rat LRRC52, respectively. LRRC-52 is dominantly expressed in testis and skeletal muscle and is also detected in several other tissues including placenta, kidney, lung, and some glands (2, 3). LRRC52 has been identified as a testes specific SLO3 K⁺ channel accessory protein in mouse (2). LRRC52 is proposed to be essential for alkalization dependent activation of the KSper current at physiological membrane potentials and pH (2). Activation of the SLO3 channel is critical in the fertilization process and loss of function results in infertility (2, 4, 5). A second Elron cluster protein, LRRC26, was recently identified as a regulatory subunit of SLO1 BK-type K⁺ channels, suggesting a general function for the entire family (6). Based on function, LRRC52 might be a therapeutic target for male contraception (7).

1. Dolan, J. et al. (2007) BMC Genomics 8:320.
2. Yang, C. et al. (2011) PNAS 108:19419.
3. Leonetti, M. et al. (2012) PNAS. 109:19274.
4. Navarro, B. et al. (2007) PNAS 104:7688.
5. Sanit, C. et al. (2010) FEBS Lett. 584:1041.
6. Yan, J. and Aldrich, RW. (2010) Nature 466:513.
7. Zeng, XH. et al. (2015) PNAS 112:2599.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q8N7C0()