LRRC3 Antibody

LRRC3 was detected in immersion fixed paraffin-embedded sections of human brain (medulla) using Sheep Anti-Human LRRC3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5039) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counter-stained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

LRRC3 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human LRRC3
  Cys33-Asp204
  Accession # Q9BY71
• Specificity: Detects human LRRC3 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross‑reactivity with recombinant human LRRC4 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: LRRC3
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LRRC3 Antibody

• C21orf102
• chromosome 21 open reading frame 102
• leucine rich repeat containing 3
• leucine-rich repeat-containing protein 3
• LRRC3
• LRRC3A

Background

LRRC3 (Leu-rich repeat/LRR-containing protein 3) is a 25 kDa (predicted) member of the Leu-rich repeat protein superfamily. Little is known about the protein except for the fact that it shows widespread expression. Human LRRC3 is a protein that is 225 amino acids (aa) in length. It contains three LRRs between aa 63‑86, aa 87‑110 and aa 112‑135. There is one potential splice variant that shows a deletion of aa 129‑248. Over aa 33‑204, human LRRC3 is 83% and 81% aa identical to mouse and canine LRRC3, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LRRC3
• Uniprot:
  • Q9BY71()