LMW E.Coli Host Cell Protein Antibody - BSA Free

Western Blot: LMW E.Coli Host Cell Protein Antibody [NBP2-44222] - 2D Western Blot of anti- E.coli Low Molecular Weight Host Cell Protein antibody. Load: 35 Ug LMW HCP.

ELISA: LMW E.Coli Host Cell Protein Antibody [NBP2-44222] - ELISA of Rabbit anti-Low Molecular Weight Host Cell Protein Antigen: BSA conjugates of LMW HCP. Coating amount: 1 Ug per well titrated 1:3 in 1XPBS.

• Reactivity: Ec
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

LMW E.Coli Host Cell Protein Antibody - BSA Free Summary

• Description: This whole rabbit antiserum was prepared by delipidation and defibrination followed by the addition of buffer salts and preservative
  
  Store this antibody at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: LMW E.Coli Host Cell Protein Antibody was prepared by repeated immunizations with a mixture low molecular weight (LMW) proteins from E.Coli (BL21).
• Isotype: Serum
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Delipidation and Defibrination

Applications/Dilutions

• Dilutions:
  • ELISA 1:1000-1:2000
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 1:500-1:1000
• Application Notes: This product detects over 270 E.coli LMW proteins and has been tested by 2D, western blotting, and ELISA.

Reactivity Notes

This whole rabbit antiserum was prepared by delipidation and defibrination followed by the addition of buffer salts and preservative. Reactivity is directed against low molecular weight E.coli Host Cell Proteins. Cross-reactivity with other species is likely but has not been determined. Reactivity against homologues from other sources is not known.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Delipidation and Defibrination

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.