![Immunohistochemistry-Paraffin: Lewis Y Antibody (LWY/9243R) - Azide and BSA Free [NBP3-24188] - Formalin-fixed, paraffin-embedded human stomach stained with Lewis Y Antibody (LWY/9243R). Inset: PBS instead of primary antibody; secondary only negative control.](http://images.novusbio.com/fullsize/nbp3-24188_rabbit-lewis-y-mab-lwy-9243r-azide-and-bsa-free-24120257592896.jpg "Immunohistochemistry-Paraffin: Lewis Y Antibody (LWY/9243R) - Azide and BSA Free [NBP3-24188] - Formalin-fixed, paraffin-embedded human stomach stained with Lewis Y Antibody (LWY/9243R). Inset: PBS instead of primary antibody; secondary only negative control.")
| Reactivity | HuSpecies Glossary |
| Applications | IHC |
| Clone | LWY/9243R |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Description | Antibody without azide - store at -20 to -80C. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | H3396 cultured line established from a metastasis of a human breast adenocarcinoma. |
| Localization | Cell membrane. Cytoplasm. Extracellular. |
| Marker | Tumor Marker |
| Isotype | IgG Kappa |
| Clonality | Monoclonal |
| Host | Rabbit |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10mM PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")