![Immunocytochemistry /Immunofluorescence: Lewis Y Antibody (H18A) - Azide and BSA Free [NBP3-09007] - Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells on Shi-fix(TM) coverslips, permeabilized with 0.15% Triton and stained with NBP3-09007 at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom: NBP3-09007, DAPI, merged channels and an isotype control. The isotype control was stained with an unknown specificity antibody followed by Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-09007_mouse-monoclonal-lewis-y-antibody-h18a-74202522141249.png "Immunocytochemistry /Immunofluorescence: Lewis Y Antibody (H18A) - Azide and BSA Free [NBP3-09007] - Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells on Shi-fix(TM) coverslips, permeabilized with 0.15% Triton and stained with NBP3-09007 at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom: NBP3-09007, DAPI, merged channels and an isotype control. The isotype control was stained with an unknown specificity antibody followed by Alexa Fluor(R) 488 secondary antibody.")
| Reactivity | HuSpecies Glossary |
| Applications | ELISA, ICC/IF, IHC |
| Clone | H18A |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Description | Novus Biologicals Mouse Lewis Y Antibody (H18A) - Azide and BSA Free (NBP3-09007) is a recombinant monoclonal antibody validated for use in IHC, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Lewis Y. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | The antibody binds specifically to LeY tetrasaccharide, a carbohydrate antigen expressed at the cell surface of selective carcinomas, including those of the colon, breast, ovary and lung. The monoclonal antibody may be of clinical use for the in vivo diagnosis and therapy of certain cancers. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Proclin 300 |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
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![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")